Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.
Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.
复制标题
地塞米松可增强嗜铬细胞瘤细胞中酪氨酸羟化酶从聚 (A)-mRNA 的翻译。
DOI：
10.1073/pnas.78.2.1269
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发表时间：
1981
影响因子：
11.1
通讯作者：
Joh,TH
中科院分区：
文献类型：
--
作者：
Baetge,EE;Kaplan,BB;Reis,DJ;Joh,TH
从大鼠嗜铬细胞瘤（PC 12）克隆细胞系中纯化多聚腺苷酸（A）-mRNA，并在网织红细胞无细胞蛋白合成系统中翻译。酪氨酸羟化酶[酪氨酸3-单加氧酶; L-酪氨酸，四氢蝶啶：氧氧化还原酶（3-羟基化），EC 1.14.16.2]通过免疫沉淀和NaDodSO 4/聚丙烯酰胺凝胶电泳从其他蛋白质中分离。酪氨酸羟化酶的分子量和相对比例，在体外合成的其他蛋白质是相同的，在体内培养的细胞中合成的酶。用10 μ M地塞米松孵育PC 12细胞可使酪氨酸羟化酶的活性和数量增加2.5倍。酪氨酸羟化酶与从用地塞米松处理的细胞分离的poly（A）-mRNA翻译的总蛋白质的比率比从来自未处理细胞的等量poly（A）-mRNA翻译的酶与总蛋白质的比率高2.5倍。因此，地塞米松引起的PC 12细胞中酪氨酸羟化酶的诱导是酪氨酸羟化酶mRNA的“相对”量或活性增加的结果。
Polysomal poly(A)-mRNA was purified from a clonal cell line of rat pheochromocytoma (PC 12) and translated in a reticulocyte cell-free protein-synthesizing system. Tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine, tetrahyropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] was isolated from other protein by immunoprecipitation and NaDodSO4/polyacrylamide gel electrophoresis. The molecular weight and relative proportion of tyrosine hydroxylase to other proteins synthesized in vitro were identical to those of the enzyme synthesized in vivo in cultured cells. Incubation of PC 12 cells with 10 microM dexamethasone increased the activity and amount of tyrosine hydroxylase 2.5-fold. The ratio of tyrosine hydroxylase to total protein translated from poly(A)-mRNA isolated from cells treated with dexamethasone was 2.5 times higher than the ratio of enzyme to total protein translated from an equal amount of poly(A)-mRNA from untreated cells. The dexamethasone-elicited induction of tyrosine hydroxylase in PC 12 cells therefore is a result of an increased "relative" amount or activity of tyrosine hydroxylase mRNA.