Improved functional recovery of denervated skeletal muscle after temporary sensory nerve innervation
Improved functional recovery of denervated skeletal muscle after temporary sensory nerve innervation
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DOI：
10.1016/s0306-4522(00)00577-7
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Fahnestock, M
中科院分区：
文献类型：
--
作者：
Bain, JR;Veltri, KL;Fahnestock, M
长时间的肌肉去神经支配会导致神经修复后功能恢复不良。在运动神经修复之前，已在大鼠中检查了去神经肌肉的暂时感觉神经支配的可能保护作用。通过切断胫神经来去除比目鱼肌和腓肠肌的神经，然后立即或在两个月、四个或六个月后将腓神经缝合到横切的远端胫神经残端上。在一半修复延迟的动物中，隐神经（感觉）神经暂时附着在远端神经残端上。在腓骨-胫骨连接三个月后对肌肉进行评估，并与未手术的对照肌肉和未治疗的去神经肌肉进行比较。经过四到六个月的感觉“保护”后，腓肠肌的重量明显大于未保护的肌肉，并且腓肠肌和比目鱼肌都表现出更好的结构保存，纤维萎缩和结缔组织增生较少。无论运动神经会合延迟如何，感觉保护后腓肠肌和比目鱼肌的最大复合动作电位显着增大。等长力虽然小于对照动物和立即神经修复的动物，但在感觉保护后保持相当恒定，而在未受保护的肌肉中，随着去神经时间的延长，等长力逐渐显着下降。我们将这些结果解释为表明，尽管感觉神经无法形成可兴奋的神经肌肉接头，但感觉神经仍然可以对去神经肌肉纤维产生强大的营养作用。我们认为这些发现表明了改善周围神经手术结果的有用策略。 (C) 2001 国际广播组织。由爱思唯尔科学有限公司出版。保留所有权利。
Prolonged muscle denervation results in poor functional recovery after nerve repair. The possible protective effect of temporary sensory innervation of denervated muscle, prior to motor nerve repair, has been examined in the rat. Soleus and gastrocnemius muscles were denervated by cutting the tibial nerve, and the peroneal nerve was then sutured to the transected distal tibial nerve stump either immediately or after two, four or six months. In half of the animals with delayed repair, the saphenous (sensory) nerve was temporarily attached to the distal nerve stump. Muscles were evaluated three months after the peroneal-to-tibial union, and were compared with each other, with unoperated control muscles and with untreated denervated muscles. After four to six months of sensory "protection", gastrocnemius muscles weighed significantly more than unprotected muscles, and both gastrocnemius and soleus muscles exhibited better preservation of their structure, with less fiber atrophy and connective tissue hyperplasia. The maximum compound action potentials were significantly larger in gastrocnemius and soleus muscles following sensory protection, irrespective of the delay in motor nerve union. Isometric force, although less than in control animals and in those with immediate nerve repair, remained reasonably constant after sensory protection, while in unprotected muscles there was a progressive and significant decline as the period of denervation lengthened.We interpret these results as showing that, although incapable of forming excitable neuromuscular junctions, sensory nerves can nevertheless exert powerful trophic effects on denervated muscle fibers. We propose that these findings indicate a useful strategy for improving the outcome of peripheral nerve surgery. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.