EXPRESSION OF C-FOS PROTEIN IN BRAIN - METABOLIC MAPPING AT THE CELLULAR-LEVEL
EXPRESSION OF C-FOS PROTEIN IN BRAIN - METABOLIC MAPPING AT THE CELLULAR-LEVEL
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DOI：
10.1126/science.3131879
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发表时间：
1988-06-03
期刊：
影响因子：
56.9
通讯作者：
CURRAN, T
中科院分区：
文献类型：
--
作者：
SAGAR, SM;SHARP, FR;CURRAN, T
原癌基因c - fos在神经元中受生长因子和神经递质直接刺激而表达。为了确定c - fos蛋白（Fos）及Fos相关蛋白是否可由多突触激活诱导产生，对大鼠后肢运动/感觉皮层进行电刺激，并通过免疫组织化学方法检测Fos的表达。刺激开始3小时后，在运动和感觉丘脑、脑桥核、苍白球和小脑中观察到局灶性核Fos染色。此外，禁水24小时导致室旁核和视上核中出现Fos表达。因此，Fos免疫组织化学提供了一种标记多突触激活神经元的细胞方法，从而绘制功能通路。
The proto-oncogene c-fos is expressed in neurons in response to direct stimulation by growth factors and neurotransmitters. In order to determine whether the c-fos protein (Fos) and Fos-related proteins can be induced in response to polysynaptic activation, rat hindlimb motor/sensory cortex was stimulated electrically and Fos expression examined immunohistochemically. Three hours after the onset of stimulation, focal nuclear Fos staining was seen in motor and sensory thalamus, pontine nuclei, globus pallidus, and cerebellum. Moreover, 24-hour water deprivation resulted in Fos expression in paraventricular and supraoptic nuclei. Fos immunohistochemistry therefore provides a cellular method to label polysynaptically activated neurons and thereby map functional pathways.