Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient.
Molecular analysis of arbuscular mycorrhizal fungi colonising a semi-natural grassland along a fertilisation gradient.
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DOI：
10.1111/j.1469-8137.2006.01799.x
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发表时间：
2006-10
期刊：
影响因子：
--
通讯作者：
Juan C. Santos;R. Finlay;A. Tehler
中科院分区：
文献类型：
--
作者：
Juan C. Santos;R. Finlay;A. Tehler
丛枝菌根真菌（AMF）的社区定居的根的羊茅和千叶苜蓿的特点是在瑞典牧场在不同的时间，沿着梯度施肥。对小亚基核糖体RNA基因进行PCR和变性梯度凝胶电泳（DGGE）、测序和系统发育分析。在这项研究中发现的序列聚集在10个离散的序列组，7个属于球囊霉属，2个属于盾孢霉属，1个属于多孢霉属。根中AMF序列组数与土壤矿质氮呈负相关。6~9月间AMF在根中的发生频率显著下降。没有植物寄主特异性可以检测到。
The community of arbuscular mycorrhizal fungi (AMF) colonizing the roots of Festuca pratensis and Achillea millefolium was characterized in a Swedish pasture at different times, along a gradient of fertilization. The small subunit ribosomal RNA gene was subjected to PCR and denaturing gradient gel electrophoresis (DGGE), sequencing and phylogenetic analysis. The sequences found in this study clustered in 10 discrete sequence groups, seven belonging to Glomus, two to Scutellospora and one to Diversispora. A negative correlation was observed between soil mineral nitrogen and the number of AMF sequence groups in the roots. The frequency of occurrence of AMF in roots decreased dramatically between June and September. No plant-host specificity could be detected.