The crk1 gene encodes an Ime2-related protein that is required for morphogenesis in the plant pathogen Ustilago maydis
The crk1 gene encodes an Ime2-related protein that is required for morphogenesis in the plant pathogen Ustilago maydis
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DOI：
10.1046/j.1365-2958.2003.03323.x
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发表时间：
2003-02-01
影响因子：
3.6
通讯作者：
Pérez-Martín, J
中科院分区：
文献类型：
--
作者：
Garrido, E;Pérez-Martín, J
真菌病原体麦氏黑穗病菌在其生命周期中交替进行出芽和丝状生长。这种二态转变受环境因素和交配的调节。我们克隆了一个新的基因，crk1，它编码的蛋白序列与酿酒酵母中参与发育选择的激酶lme2相似。在美国，crk1基因的破坏导致细胞不能以适当的方式对环境刺激做出反应，并在形态发生和细胞周期调整方面表现出缺陷，以适应不断变化的条件。我们分析了crk1基因的调控，并证明cAMP和MAPK通路对crk1的转录水平有相反的影响。此外，我们已经表明，这些途径的组成部分的改变损害了细胞机制适应变化条件的能力。这些结果表明，crk1编码蛋白在黑穗病菌的形态发生和环境适应中起着重要作用。
The fungal pathogen Ustilago maydis alternates between budding and filamentous growth during its life cycle. This dimorphic transition is regulated by environmental factors and mating. We cloned a new gene, crk1, which encodes a protein with sequence similarity to lme2, a kinase involved in developmental choices in S. cerevisiae. Disruption of the crk1 gene in U. maydis resulted in cells that are unable to respond in an appropriate manner to environmental stimuli and show defects in morphogenesis and cell cycle adjustment to changing conditions. We have analysed the regulation of the crk1 gene and demonstrated that cAMP and MAPK pathways have opposite influences on the transcript levels for crk1. Furthermore, we have shown that alterations in the components of these pathways impair the ability of the cellular machinery to adapt to changing conditions. These results demonstrate an important role for the crk1-encoded protein in the morphogenesis and environmental adaptation in Ustilago maydis.