EXPRESSION OF INTERLEUKIN-1-ALPHA AND INTERLEUKIN-BETA IN EARLY-PASSAGE FIBROBLASTS FROM AGING INDIVIDUALS
EXPRESSION OF INTERLEUKIN-1-ALPHA AND INTERLEUKIN-BETA IN EARLY-PASSAGE FIBROBLASTS FROM AGING INDIVIDUALS
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DOI：
10.1016/0531-5565(93)90039-g
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发表时间：
1993-11-01
影响因子：
3.9
通讯作者：
BAGLIONI, C
中科院分区：
文献类型：
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作者：
KUMAR, S;VINCI, JM;BAGLIONI, C
来自新生儿包皮的人二倍体成纤维细胞 (HDF) 在完成至少 70%（几乎等于 40 次群体倍增）的体外寿命后，会持续表达白细胞介素 1 (IL-1) mRNA 和蛋白质。 这种 IL-1 反过来会诱导老化 HDF 中特定蛋白质的合成。为了确定体内衰老是否会促进 IL-1 表达，我们分析了两个 55 岁和 92 岁正常个体的 HDF 以及一名由维尔纳综合征引起的过早衰老患者的 HDF 中 IL-1 和可诱导 mRNA 的表达。通过逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA），我们检测了正常个体和维尔纳综合征患者的早期传代HDF中IL-1α和β mRNA和蛋白的表达。这些 HDF 还表达基质溶解素、纤溶酶原激活剂抑制剂 2 型、锰超氧化物歧化酶和胶原酶的 IL-1 诱导 mRNA。这些结果表明，IL-1 的年龄依赖性表达发生在体内或体外几次细胞分裂后。因此，成纤维细胞表型在衰老过程中通过 IL-1 诱导基因的表达而改变。
Human diploid fibroblasts (HDFs) from newborn foreskin constitutively express interleukin-1 (IL-1) mRNA and protein after completing at least 70% (almost-equal-to 40 population doublings) of their in vitro life span. This IL-1 in turn induces the synthesis of specific proteins in aging HDFs. To determine whether IL-1 expression may be promoted by in vivo aging, we analyzed the expression of IL-1 and of inducible mRNAs in HDFs from two normal individuals 55 and 92 years old and in HDFs from a patient with premature aging caused by Werner's syndrome. By reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), we detected expression of IL-1alpha and beta mRNA and protein in early passage HDFs from both normal individuals and the Werner's syndrome patient. These HDFs also expressed the IL-1-inducible mRNAs for stromelysin, plasminogen activator inhibitor type 2, manganous superoxide dismutase, and collagenase. These results suggest that an age-dependent expression of IL-1 occurs either in vivo or after a few cell divisions in vitro. Therefore, the fibroblast phenotype is modified by the expression of IL-1-inducible genes during aging.