Proteomic analysis of middle and late stages of bread wheat (Triticum aestivum L.) grain development.
Proteomic analysis of middle and late stages of bread wheat (Triticum aestivum L.) grain development.
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DOI：
10.3389/fpls.2015.00735
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发表时间：
2015
影响因子：
5.6
通讯作者：
Cui D
中科院分区：
文献类型：
--
作者：
Zhang N;Chen F;Huo W;Cui D
将蛋白质组学方法应用于中国面包小麦豫农201及其甲磺酸乙酯（EMS）突变系豫农3114的四个籽粒发育阶段。利用2-DE和串联MALDI-TOF/TOF-MS分析了中国面包小麦豫农201及其较大粒级EMS突变系豫农3114在籽粒发育中后期的蛋白质组特征。我们鉴定了代表 88 种独特蛋白质的 130 个差异积累的蛋白质点，四种主要表达模式显示了中后期籽粒形成的动态描述。这些确定的蛋白质种类参与八个生化过程：应激/防御、碳水化合物代谢、蛋白质合成/组装/降解、储存蛋白质、能量产生和运输、光合作用、转录/翻译、信号转导。比较蛋白质组学表征表明，两个小麦品种中共积累了12个具有不同表达模式的蛋白点，以及6个品种特异性蛋白点，包括丝氨酸蛋白酶抑制剂、小热休克蛋白、β-淀粉酶、α-淀粉酶抑制剂、二聚体α-淀粉酶抑制剂前体和冷调节蛋白。这些品种特异性蛋白点可能导致两个小麦品种的产量相关性状差异。我们的结果为剖析面包小麦产量相关性状的分子和遗传学基础提供了有价值的信息，本研究中的蛋白质组表征还可以为中后期谷物发育的生物学提供见解。
Proteomic approaches were applied in four grain developmental stages of the Chinese bread wheat Yunong 201 and its ethyl methanesulfonate (EMS) mutant line Yunong 3114. 2-DE and tandem MALDI-TOF/TOF-MS analyzed proteome characteristics during middle and late grain development of the Chinese bread wheat Yunong 201 and its EMS mutant line Yunong 3114 with larger grain sizes. We identified 130 differentially accumulated protein spots representing 88 unique proteins, and four main expression patterns displayed a dynamic description of middle and late grain formation. Those identified protein species participated in eight biochemical processes: stress/defense, carbohydrate metabolism, protein synthesis/assembly/degradation, storage proteins, energy production and transportation, photosynthesis, transcription/translation, signal transduction. Comparative proteomic characterization demonstrated 12 protein spots that co-accumulated in the two wheat cultivars with different expression patterns, and six cultivar-specific protein spots including serpin, small heat shock protein, β-amylase, α-amylase inhibitor, dimeric α-amylase inhibitor precursor, and cold regulated protein. These cultivar-specific protein spots possibly resulted in differential yield-related traits of the two wheat cultivars. Our results provide valuable information for dissection of molecular and genetics basis of yield-related traits in bread wheat and the proteomic characterization in this study could also provide insights in the biology of middle and late grain development.