Conformational changes in the archaerhodopsin-3 proton pump: detection of conserved strongly hydrogen bonded water networks
Conformational changes in the archaerhodopsin-3 proton pump: detection of conserved strongly hydrogen bonded water networks
复制标题
archaerhodopsin-3 质子泵的构象变化：检测保守的强氢键水网络
DOI：
10.1007/s10867-011-9246-4
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发表时间：
2012
影响因子：
1.8
通讯作者：
K. Rothschild
中科院分区：
文献类型：
--
作者：
Erica C. Saint Clair;John I. Ogren;S. Mamaev;Joel M. Kralj;K. Rothschild
古视紫红质-3（AR 3）是一种光驱动的质子泵，但对其光循环机制知之甚少。最近的兴趣集中在AR 3，因为它的能力，既作为一个高性能的，遗传靶向的神经元活动的光学沉默器，并作为膜电压传感器。我们研究了光激活的蛋白质，视网膜发色团，和内部水分子的结构变化在光循环的AR 3。低温和快速扫描时间分辨FTIR-差光谱显示，在形成的K，M和N光循环中间体的构象变化是相似的，虽然不相同，细菌视紫红质（BR）。在3，600 cm − 1以上区域的正/负带，先前被认为是弱氢键内部水分子的结构变化，在AR 3和BR之间有很大的不同。这包括最近与细胞质通道中的质子转运水分子链和弱氢键水（W 401）相关的阳性条带的缺失，该弱氢键水是位于视网膜席夫碱附近的氢键五边形簇的一部分。然而，BR中许多低于3，000 cm − 1的宽红外连续吸收变化被分配给参与质子通过细胞质和细胞外部分转运的水分子网络，这些变化在AR 3中非常相似。这项工作以及随后比较BR和AR 3结构变化的研究将有助于识别BR样质子泵中的保守元件，以及生物工程AR 3，以优化神经沉默和电压传感。
Archaerhodopsin-3 (AR3) is a light-driven proton pump from Halorubrum sodomense, but little is known about its photocycle. Recent interest has focused on AR3 because of its ability to serve both as a high-performance, genetically-targetable optical silencer of neuronal activity and as a membrane voltage sensor. We examined light-activated structural changes of the protein, retinal chromophore, and internal water molecules during the photocycle of AR3. Low-temperature and rapid-scan time-resolved FTIR-difference spectroscopy revealed that conformational changes during formation of the K, M, and N photocycle intermediates are similar, although not identical, to bacteriorhodopsin (BR). Positive/negative bands in the region above 3,600 cm − 1, which have previously been assigned to structural changes of weakly hydrogen bonded internal water molecules, were substantially different between AR3 and BR. This included the absence of positive bands recently associated with a chain of proton transporting water molecules in the cytoplasmic channel and a weakly hydrogen bonded water (W401), which is part of a hydrogen-bonded pentagonal cluster located near the retinal Schiff base. However, many of the broad IR continuum absorption changes below 3,000 cm − 1 assigned to networks of water molecules involved in proton transport through cytoplasmic and extracellular portions in BR were very similar in AR3. This work and subsequent studies comparing BR and AR3 structural changes will help identify conserved elements in BR-like proton pumps as well as bioengineer AR3 to optimize neural silencing and voltage sensing.
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影响因子：
2.9
作者：
BRAIMAN, MS;MOGI, T;ROTHSCHILD, KJ
通讯作者：
ROTHSCHILD, KJ
影响因子：
56.9
作者：
Luecke, H;Schobert, B;Lanyi, JK
通讯作者：
Lanyi, JK
细菌视紫红质 M-中间体形成时苏氨酸结构变化的检测：分配给 Thr-89 的证据。
DOI：
10.1016/s0005-2728(98)00088-7
发表时间：
1998
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Liu,X;Lee,MJ;Coleman,M;Rath,P;Nilsson,A;Fischer,WB;Bizounok,M;Herzfeld,J;Karstens,WF;Raap,J;Lugtenburg,J;Rothschild,KJ
通讯作者：
Rothschild,KJ
影响因子：
2.9
作者：
Dioumaev, AK;Brown, LS;Lanyi, JK
通讯作者：
Lanyi, JK
精氨酸 82 在细菌视紫红质光循环过程中快速质子释放中的作用：对含有 15N 标记精氨酸的紫色膜进行时间分辨 FT-IR 研究。
DOI：
10.1021/bi049238g
发表时间：
2004
期刊：
Biochemistry.
影响因子：
--
作者：
Xiao,Yaowu;Hutson,MShane;Belenky,Marina;Herzfeld,Judith;Braiman,MarkS
通讯作者：
Braiman,MarkS