Effects of the vitamin E in catalase activities in hippocampus after status epilepticus induced by pilocarpine in Wistar rats
Effects of the vitamin E in catalase activities in hippocampus after status epilepticus induced by pilocarpine in Wistar rats
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DOI：
10.1016/j.neulet.2007.01.057
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发表时间：
2007-04-18
影响因子：
2.5
通讯作者：
Takahashi, R. N.
中科院分区：
文献类型：
--
作者：
Barros, D. O.;Xavier, S. M. L.;Takahashi, R. N.
实验操作表明，体内施用外源性抗氧化剂可降低大脑中自由基的浓度。神经化学研究已经提出过氧化氢酶在由匹罗卡平诱导的癫痫持续状态（SE）发展的脑机制中的作用。本研究旨在探讨匹罗卡品诱导SE后过氧化氢酶活性的变化。动物腹腔注射维生素E (VIT E) 200 mg/kg， 30 min后皮下注射盐酸匹罗卡平400 mg/kg。(P400)。其余3组单独给予VIT E （200 mg/kg, i.p）、匹罗卡品（400 mg/kg, s.c）或0.9% NaCl（对照）。在注射匹罗卡品24小时内密切观察动物的行为改变、震颤、刻板动作、癫痫发作、SE和死亡。头颅被砍头后，大脑被解剖。结果表明，匹罗卡品给药和由此产生的SE使海马过氧化氢酶活性显著增加（88%）。预处理组海马过氧化氢酶活性VIT E分别较P400组和对照组提高67%和214%。我们的研究结果直接证明了成年大鼠在癫痫发作期间和预处理后海马过氧化氢酶活性的增加，而VIT E可能是在SE建立期间调节自由基水平的原因。2007爱思唯尔爱尔兰有限公司版权所有。
Experimental manipulations suggest that in vivo administration of exogenous antioxidants agents decreases the concentration of free radical in the brain. Neurochemical studies have proposed a role for catalase in brain mechanisms responsible by development to status epilepticus (SE) induced by pilocarpine. The present study was aimed at was investigating the changes in catalase activities after pilocarpine-induced SE. Animals were treated with vitamin E (VIT E) 200 mg/kg (intraperitoneally (i.p.)) and, 30 min later, they received pilocarpine hydrochloride, 400 mg/kg, subcutaneous (s.c.) (P400). Other three groups received VIT E (200 mg/kg, i.p.), pilocarpine (400 mg/kg, s.c.) or 0.9% NaCl (control) alone. Animals were closely observed for behavioral changes, tremors, stereotyped movements, seizures, SE and death, for 24 h following the pilocarpine injection. The brains were dissected after decapitation. The results have shown that pilocarpine administration and resulting SE produced a significant increase in hippocampal catalase activity of (88%). In the group pre-treated which VIT E in hippocampal catalase activity was increase of 67% and 214% when compared with P400 and control group, respectively. Our results demonstrated a direct evidence of an increase in the activity of the hippocampal catalase of rat adults during seizure activity and after the pre-treated which VIT E that could be responsible by regulation of free radical levels during the establishment of SE. (c) 2007 Elsevier Ireland Ltd. All rights reserved.