Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4
Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4
复制标题
DOI：
10.1242/dev.00925
复制
发表时间：
2004-03-01
期刊：
影响因子：
4.6
通讯作者：
Berleth, T
中科院分区：
文献类型：
--
作者：
Hardtke, CS;Ckurshumova, W;Berleth, T
生长素反应因子（ARF）家族的转录因子参与了植物生长素依赖的基因调控，但对植物中单个ARF的功能知之甚少。在这里，相互作用试验，表达研究和多个功能丧失和获得突变体的组合被用来评估两个ARF，非光致性下胚轴4（NPH 4/ARF 7）和单翅目（MP/ARF 5），在拟南芥发育中的作用。MP和NPH 4两者都与自身和彼此强烈地和选择性地相互作用，并且在非常重叠的结构域中表达。我们表明，这两个基因的调控特性是远远超过其单一突变表型所建议的。NPH 4和MP能够控制胚中轴的形成和生长素依赖的细胞扩增。W和NPH 4在拟南芥植物中的相互作用是由它们在一些生长素反应中的联合需求和与两个基因的共过表达相关的协同效应来指示的。最后，我们证明了ARF和Aux/IAA基因功能在拟南芥发育中的拮抗相互作用。MP的过表达抑制了与BODENLOS（BDL）/IAA 12中的功能获得性突变相关的许多缺陷。总之，这些结果提供了证据的ARF-ARF和ARF-Aux/IAA在拟南芥植物中的相互作用的生物学相关性，并表明，一个单独的ARF可以在两个不变的编程模式的形成，以及在条件响应外部信号。
Transcription factors of the auxin response factor (ARF) family have been implicated in auxin-dependent gene regulation, but little is known about the functions of individual ARFs in plants. Here, interaction assays, expression studies and combinations of multiple loss- and gain-of-function mutants were used to assess the roles of two ARFs, NONPHOTOTROPIC HYPOCOTYL 4 (NPH4/ARF7) and MONOPTEROS (MP/ARF5), in Arabidopsis development. Both MP and NPH4 interact strongly and selectively with themselves and with each other, and are expressed in vastly overlapping domains. We show that the regulatory properties of both genes are far more related than suggested by their single mutant phenotypes. NPH4 and MP are capable of controlling both axis formation in the embryo and auxin-dependent cell expansion. Interaction of W and NPH4 in Arabidopsis plants is indicated by their joint requirement in a number of auxin responses and by synergistic effects associated with the co-overexpression of both genes. Finally, we demonstrate antagonistic interaction between ARF and Aux/IAA gene functions in Arabidopsis development. Overexpression of MP suppresses numerous defects associated with a gain-of-function mutation in BODENLOS (BDL)/IAA12. Together these results provide evidence for the biological relevance of ARF-ARF and ARF-Aux/IAA interaction in Arabidopsis plants and demonstrate that an individual ARF can act in both invariantly programmed pattern formation as well as in conditional responses to external signals.