HISTOCHEMISTRY OF GLYCOGEN DEPOSITION IN PERINATAL RAT-BRAIN - IMPORTANCE OF RADIAL GLIAL-CELLS
HISTOCHEMISTRY OF GLYCOGEN DEPOSITION IN PERINATAL RAT-BRAIN - IMPORTANCE OF RADIAL GLIAL-CELLS
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DOI：
10.1007/bf01262651
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
BIESOLD, D
中科院分区：
文献类型：
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作者：
BRUCKNER, G;BIESOLD, D
用高碘酸-希夫法研究了大鼠胚胎（艾德）14、16、18、20天和出生后（PD）1、3、7、12、16、21天脑内糖原含量和沉积模式的变化。区域和时间的差异进行量化与自动图像分析仪通过估计染色材料在软膜下区域的大脑皮层，丘脑，上级丘和髓质。在艾德18、艾德20和PD 2上通过电子显微镜研究糖原颗粒的细胞定位。在艾德14日，在部分未成熟的脉络丛和中脑和延髓中缝的放射状胶质细胞中发现了糖原储存的最初迹象。随着胎龄的增加，这些结构保留了其高糖原储存能力，但除此之外，大部分脑干区域和较大胎龄（艾德18-20）大脑皮质中放射状纤维数量增加，显示出大量糖原。在研究的所有胎龄间隔，大脑皮质中糖原的储存相对较低。在新生动物中，糖原颗粒的分布与晚期胎儿大脑中发现的分布相似。糖原含量从PD 1 ~ 3开始下降，在PD 7 ~ 21达到成年期的水平。放射状胶质细胞在发育中储存糖原可能是围产期糖代谢的能量来源。
Changes in the glycogen content and patterns of deposition in the developing rat brain were studied using a light microscopic periodic acid-Schiff method on embryonic days (ED) 14, 16, 18, 20 and postnatal days (PD) 1, 3, 7, 12, 16 and 21. Regional and temporal differences were quantified with an automatic image analyser by estimation of stained material in subpial regions of cerebral cortex, thalamus, superior colliculus and medulla. The cellular localization of glycogen particles was investigated by electron microscopy on ED 18, ED 20 and PD 2. On ED 14 the first signs of glycogen storage were found in parts of the immature choroid plexus and in radial glial cells in the midbrain and medullary raphé. With advancing foetal age these structures retained their high capacity for glycogen storage but, in addition, an increasing number of radial fibres in most of the brain stem regions and in the cerebral cortex of older foetuses (ED 18–20) showed significant amounts of glycogen. The storage of glycogen in cerebral cortex was relatively low at all foetal age intervals studied. In new born animals the distribution of glycogen particles was similar to that found in the late foetal brain. A decrease of glycogen content commenced from PD 1 to 3 and reached the pattern of the adult brain between PD 7 and 21.Glycogen storage by radial glial cells in the developing rat brain might indicate their possible role as an energy source in perinatal carbohydrate metabolism.