Daily restricted feeding resets the circadian clock in the suprachiasmatic nucleus of CS mice
Daily restricted feeding resets the circadian clock in the suprachiasmatic nucleus of CS mice
复制标题
DOI：
10.1152/ajpregu.00331.2006
复制
发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Honma, Ken-ichi
中科院分区：
文献类型：
--
作者：
Abe, Hiroshi;Honma, Sato;Honma, Ken-ichi
研究了CS小鼠和C57BL/6J小鼠视交叉上核（SCN）生物钟基因在连续黑暗（DD）条件下的昼夜节律变化，并检测了SCN生物钟起搏器对RF的影响。在光照-黑暗循环下自由进食2-3周后，将动物释放到DD，每天固定时间饲喂3小时，持续3-4周。随后，他们恢复了2-3周的免费食物供应。在CS小鼠中，滚轮运动节律随射频运动开始与进食时间呈稳定的相位关系，在射频运动终止后，滚轮运动节律从进食时间开始自由运行。SCN中的mPer1、mPer2和mBMAL1 mRNA节律与摄食时间呈固定相位关系，表明SCN中的昼夜节律起搏器受RF影响。另一方面，在C57BL/6J小鼠中，轮跑节律在RF下自由运行，SCN中的时钟基因表达节律与摄食时间无关，而与行为节律相关，表明SCN中的昼夜节律起搏器不携带。这些结果表明，在DD的情况下，CS小鼠的SCN昼夜节律起搏器可被RF诱导，并表明CS小鼠具有可通过与进食时间相关的信号重置的昼夜节律时钟系统。
Circadian rhythms in clock gene expressions in the suprachiasmatic nucleus (SCN) of CS mice and C57BL/6J mice were measured under a daily restricted feeding (RF) schedule in continuous darkness (DD), and entrainment of the SCN circadian pacemaker to RF was examined. After 2-3 wk under a light-dark cycle with free access to food, animals were released into DD and fed for 3 h at a fixed time of day for 3-4 wk. Subsequently, they returned to having free access to food for 2-3 wk. In CS mice, wheel-running rhythms entrained to RF with a stable phase relationship between the activity onset and feeding time, and the rhythms started to free run from the feeding time after the termination of RF. mPer1, mPer2, and mBMAL1 mRNA rhythms in the SCN showed a fixed phase relationship with feeding time, indicating that the circadian pacemaker in the SCN entrained to RF. On the other hand, in C57BL/6J mice, wheel-running rhythms free ran under RF, and clock gene expression rhythms in the SCN showed a stable phase relation not to feeding time but to the behavioral rhythms, indicating that the circadian pacemaker in the SCN did not entrain. These results indicate that the SCN circadian pacemaker of CS mice is entrainable to RF under DD and suggest that CS mice have a circadian clock system that can be reset by a signal associated with feeding time.