Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases
Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases
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DOI：
10.1002/cbic.200500334
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发表时间：
2006-01-01
期刊：
影响因子：
3.2
通讯作者：
Tawfik, DS
中科院分区：
文献类型：
--
作者：
Khersonsky, O;Tawfik, DS
5-硫代烷基丁内酯（1）被证明是用于测定内酯酶活性，特别是血清对氧磷酶（PON 1）活性的有用的显色/荧光探针。这些新型探针适用于测量复杂生物样品中的内酯酶活性，包括血清、完整细胞和细胞裂解物。
5-Thioalkyl butyrolactones (1) are shown to be useful chromogenic/fluorogenic probes for determining lactonase activities, in particular of serum paraoxonase (PON1). These novel probes are suitable for measuring lactonase activity in complex biological samples, including sera, intact cells and cell lysates.