Ovulation in the perfused rabbit ovary: the influence of prostaglandins and prostaglandin inhibitors.
Ovulation in the perfused rabbit ovary: the influence of prostaglandins and prostaglandin inhibitors.
复制标题
灌注兔卵巢中的排卵：前列腺素和前列腺素抑制剂的影响。
作者：
Y. Hamada;R. Bronson;K. Wright;E. Wallach
在离体灌注兔卵巢中实现了体外排卵。HCG给药后8 h，将卵巢动脉插管，灌注并取出卵巢进行体外研究。研究了灌流液中添加PGE 2、PGF 20、消炎痛和绒毛膜促性腺激素对排卵的影响。对照组（无药物）12个卵巢中8个排卵，补充HCG（1 IU/ml）组12个卵巢中7个排卵，PGF_（2a）（1 mg/ml）组12个卵巢中11个排卵，PGE_（21）（1 mg/ml）组12个卵巢中3个排卵，吲哚美辛（0.5ug/ml）组12个卵巢中2个排卵。排卵总是发生在对侧卵巢，其保持原位作为体内对照。体外排卵实验中，灌注液中加入吲哚美辛组排卵率明显低于对照组（P<0.01）。当用PGE 2处理的卵巢与对照相比时，排卵也减少（P<0.01），当PGE 2与PGF 20相比时，排卵也减少（P<0.005）。这些观察结果表明，卵巢内PGF 20的存在可能是排卵过程所必需的。吲哚美辛和PGE 2对体外灌注兔卵巢排卵的影响与以前在完整HCG处理兔中的观察结果平行。灌注制剂的使用提供了额外的证据，即吲哚美辛和PGE 2对排卵的抑制作用是在卵巢水平介导的。同时记录卵巢收缩，但收缩模式、排卵或排卵点数量之间无关联。
Ovulation has been achieved in Vitro in the isolated perfused rabbit ovary. The ovarian artery was cannulated 8 h after HCG administration and the ovary perfused and removed for in vitro study. The effects on ovulation of the addition of PGE2, and PGF20, indomethacin and HCG to the perfusate were studied. Ovulation occurred in 8 of 12 perfused ovaries in the control group (no drug), 7 of 12 in the group perfused with supplementary HCG (1 lU/mI), 11 of 12 with PGF2a (1 Mg/mI), 3 of 12 with PGE2 (1 Mg/mI) and 2 of 12 with indomethacin (0.5 ug/mI). Ovulation always occurred in the contralateral ovary which remained in situ as an in vivo control. Addition of indomethacin to the perfusate reduced the incidence of ovulation in vitro when compared to the control group (P<0.01). Ovulation was also reduced (P<0.01) when the ovaries treated with PGE2 were compared to the control, and when PGE2 was compared to PGF20 (P<0.005). These observations indicate that the presence of PGF20 within the ovary may be essential to the process of ovulation. of indomethacin and PGE2 on ovulation in the perfused in Vitro rabbit ovary parallel previous observations in the intact HCG-treated rabbit. The use of the perfused preparation provides additional evidence that the inhibitory effects of indomethacin and PGE2 on ovulation are mediated at the ovarian level. Simultaneous recordings of ovarian contractions were carried out and no association could be established between contractile pattern, ovulation or number of ovulation points.