MAPPING OF DNASE I-HYPERSENSITIVE SITES IN THE UPSTREAM DNA OF HUMAN-EMBRYONIC EPSILON-GLOBIN GENE IN K562 LEUKEMIA-CELLS
MAPPING OF DNASE I-HYPERSENSITIVE SITES IN THE UPSTREAM DNA OF HUMAN-EMBRYONIC EPSILON-GLOBIN GENE IN K562 LEUKEMIA-CELLS
复制标题
DOI：
10.1073/pnas.81.9.2718
复制
发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
LONDON, IM
中科院分区：
文献类型：
--
作者：
TUAN, D;LONDON, IM
ε-DNA酶I-超敏感位点周围在人白血病细胞系K562和HL 60中定位了珠蛋白和c-myc基因。在K562细胞中，ε-在珠蛋白基因转录过程中，在上游侧翼的6个DNA酶（kb）中发现了6个DNase I超敏感位点，在表达c-myc基因的HL 60细胞中，在上游2kb的DNA中发现了2个DNase I超敏感位点。也不是不活跃的ε- HL 60细胞中的珠蛋白基因和K562细胞中失活的c-myc基因都显示了这样的上游DNase I敏感位点。这一结果与先前的研究一致，即在其他表达基因的5“末端的1 kb内存在DNase I超敏感位点。发现在表达的ε-DNA的更上游的位点显示出甚至更高的DNA酶I敏感性。珠蛋白和c-myc基因。在所表达的ε-DNase的6个DNA酶I超敏感位点中，在K562细胞中，最敏感的位点位于ε-珠蛋白基因上游约6 kb处。珠蛋白基因当与ε上游的DNA序列相关时，在珠蛋白基因中，发现该位点对应于包含28个连续T的延伸、3个增强子核心样序列和连续（C-A）15（T-A）6个交替嘌呤和嘧啶碱基上的延伸的区域。- 用于ε的增强子元件，珠蛋白基因的表达可能存在于该DNA酶I超敏感位点内。
The DNase I-hypersensitive sites around the .epsilon.-globin and c-myc genes were mapped in 2 human leukemia cell lines K562 and HL60. In K562 cells in which the .epsilon.-globin gene is transcribed, 6 DNase I-hypersensitive sites are found in 6 kilobases (kb) of upstream flanking DNA; in HL60 cells in which the c-myc gene is expressed, 2 DNase I-hypersensitive sites are observed in 2 kb of upstream DNA. Neither the inactive .epsilon.-globin gene in HL60 cells nor the inactive c-myc gene in K562 cells displays such upstream DNase I-hypersensitive sites. The results are consistent with previous studies that show DNase I-hypersensitive sites within 1 kb of the 5'' end of other expressed genes. Sites were found displaying even more DNase I sensitivity further upstream of expressed .epsilon.-globin and c-myc genes. Among the 6 DNase I-hypersensitive sites of the expressed .epsilon.-globin gene in K562 cells, the most sensitive site is located about 6 kb upstream of the .epsilon.-globin gene. When correlated with the DNA sequence upstream of the .epsilon.-globin gene, this site was found to correspond to a region that contains a stretch of 28 consecutive Ts, 3 enhancer core-like sequences, and a stretch on consecutive (C-A)15(T-A)6 alternating purine and pyrimidine bases. An enhancer element for .epsilon.-globin gene expression may reside within this DNase I-hypersensitive site.