Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells
Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells
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DOI：
10.1002/jor.22005
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Kaps, Christian
中科院分区：
文献类型：
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作者：
Krueger, Jan Philipp;Hondke, Sylvia;Kaps, Christian
在软骨修复中，富血小板血浆（PRP）用于一步法，利用微骨折和基质诱导的软骨形成程序，骨髓源性细胞移植或关节内注射。本研究的目的是评估人PRP对人软骨下祖细胞迁移和软骨分化的影响。人祖细胞来源于软骨下皮质海绵骨（CSP），在96孔趋化性测定中分析PRP处理后的迁移能力，并在5% PRP存在下在无血清条件下在高密度团块培养物中培养。趋化性实验表明，0.1100% PRP对小鼠的趋化性有显著影响（p？
In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96-well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1100% PRP significantly (p?