Evaluation of the Metabolic Effects of Hydrogen Sulfide on the Development of Bombyx mori (Lepidoptera: Bombycidae), Using Liquid Chromatography-Mass Spectrometry-Based Metabolomics
Evaluation of the Metabolic Effects of Hydrogen Sulfide on the Development of Bombyx mori (Lepidoptera: Bombycidae), Using Liquid Chromatography-Mass Spectrometry-Based Metabolomics
复制标题
使用基于液相色谱-质谱的代谢组学评估硫化氢对家蚕（鳞翅目：家蚕科）发育的代谢影响
DOI：
10.1093/jisesa/ieaa008
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发表时间：
2020-03-14
影响因子：
2.2
通讯作者：
Wei, Zhao-Jun
中科院分区：
文献类型：
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作者：
Cao, Yu-Yao;Peng, Li-Li;Wei, Zhao-Jun
硫化氢（H2S）是一种剧毒气体，有臭鸡蛋的臭味。以往对H2S的研究主要集中在其对哺乳动物神经和呼吸系统的影响上。本研究采用基于液相色谱-质谱（LC-MS）的血淋巴代谢组学方法研究了家蚕在H2S暴露下的发育参数和代谢物的变化。H2S暴露后，雏菊的发育参数（体重、茧重、茧壳重和茧壳比）均显著增加，其中在7.5 μ m H2S下影响最大。H2S暴露（7.5 μM）下代谢产物上调，包括(6Z, 9Z, 12Z)-十八碳三烯酸、磷酸胆碱和苹果酸，而十六烷酸下调。所鉴定的代谢物涉及生物过程，包括嘧啶、嘌呤和脂肪酸代谢，这些代谢可能影响丝腺功能。这些结果表明H2S有利于家蚕的发育，并改变与纺丝功能和炎症相关的代谢途径。本研究为H2S在昆虫体内的潜在功能以及与此现象相关的代谢途径提供了新的信息。
Abstract Hydrogen sulfide (H2S) is a highly poisonous gas with an unpleasant smell of rotten eggs. Previous studies of H2S have primarily focused on its effects on mammalian nervous and respiratory systems. In this study, silkworm developmental parameters and changes in metabolites in response to H2S exposure were investigated using a hemolymph metabolomic approach, based on liquid chromatography–mass spectrometry (LC-MS). The developmental parameters, body weight, cocoon weight, cocoon shell weight, and cocoon shell ratio, were noticeably increased following H2S exposure, with the greatest effects observed at 7.5-μM H2S. Metabolites upregulated under H2S exposure (7.5 μM) were related to inflammation, and included (6Z, 9Z, 12Z)-octadecatrienoic acid, choline phosphate, and malic acid, while hexadecanoic acid was downregulated. Identified metabolites were involved in biological processes, including pyrimidine, purine, and fatty acid metabolism, which are likely to affect silk gland function. These results demonstrate that H2S is beneficial to silkworm development and alters metabolic pathways related to spinning function and inflammation. The present study provides new information regarding the potential functions of H2S in insects and metabolic pathways related to this phenomenon.