THE INTERACTION OF PROTEINS WITH HYDROXYAPATITE .1. ROLE OF PROTEIN CHARGE AND STRUCTURE
THE INTERACTION OF PROTEINS WITH HYDROXYAPATITE .1. ROLE OF PROTEIN CHARGE AND STRUCTURE
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DOI：
10.1016/0003-2697(84)90239-2
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
GORBUNOFF, MJ
中科院分区：
文献类型：
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作者：
GORBUNOFF, MJ
从羟基磷灰石柱洗脱蛋白质的标准进行了检查，作为蛋白质等电点的函数（22种蛋白质，等电点在3.5和11.0之间）;洗脱液的离子性质（PO 4，F-，Cl-，SCN-，ClO 4-和CaCl 2的钠盐）;和相关蛋白质之间的结构差异。蛋白质可分为3类：碱性蛋白质，其具有相似的中等摩尔浓度的PO 4、F-、Cl-、SCN-和ClO 4-，（< 0.003 M）Ca ~（2+）：酸性蛋白质，能与中等摩尔浓度的PO ~（4+）和F ~-结合，但不与Ca ~（2+）结合，通常也不与Cl ~-结合;中性蛋白质与PO_4、F ~-、Cl ~-结合，但对Ca ~（2+）、SCN ~-不结合，对阴离子有较强的专一性。一般而言，单个特定极性基团对结合或解吸并不重要，并且除了严重松动之外，结构变化不会强烈影响蛋白质-羟基磷灰石相互作用的模式。
The criteria for elution of proteins from hydroxyapatite columns were examined as a function of protein isoelectric point (22 proteins with isoelectric points between 3.5 and 11.0); ionic nature of eluant (Na salts of PO4, F-, Cl-, SCN-, ClO4- and CaCl2); and structural differences between related proteins. Proteins can be classified into 3 groups: basic proteins, which elute at similar, moderate molarities of PO4, F-, Cl-, SCN- and ClO4- and low (< 0.003 M) Ca2+; acidic proteins which elute at about equal moderate molarities of PO4 and F-, but do not elute with Ca2+ and usually not with Cl-; and neutral proteins, which elute with PO4, F- and Cl-, but show a strong anion specificity, and do not elute with Ca2+ or SCN-. Individual specific polar groups are not, in general, crucial to binding or desorption, and variations in structure, other than major loosening, do not strongly influence the pattern of protein-hydroxyapatite interaction.