THE AUTOMATED MEASUREMENT OF HINDLIMB FLEXOR REFLEX OF THE RAT AS A SUBSTITUTE FOR THE TAIL-FLICK ASSAY
THE AUTOMATED MEASUREMENT OF HINDLIMB FLEXOR REFLEX OF THE RAT AS A SUBSTITUTE FOR THE TAIL-FLICK ASSAY
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DOI：
10.1016/0165-0270(88)90035-0
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发表时间：
1988-05-01
影响因子：
3
通讯作者：
GYBELS, J
中科院分区：
文献类型：
--
作者：
DUYSENS, J;GYBELS, J
本文介绍了一种研究刺激镇痛的新方法。后肢屈肌肌电图，在响应于有害的电刺激的爪子，被用作间接指标的镇痛诱导的电刺激在不同的大脑部位。在轻度麻醉的大鼠（戊巴比妥10-20 mg/kg/h）中，这种反应相当稳定，并且在短至10 s的试验间隔内容易诱发，而不会形成习惯。高重复率允许快速确定抑制后肢退缩所需的阈值电流强度或大脑刺激。该测试是客观的，避免了皮肤损伤和致敏，并且可以在使用小型PC执行时半自动执行。
A new method for the study of stimulation-produced analgesia is introduced. The hindlimb flexor EMG, in response to noxious electrical stimulation of the paw, is used as an indirect index of analgesia induced by electrical stimulation at different brain sites. In the lightly anesthetized rat (pentobarbital 10-20 mg/kg/h) such responses are quite stable and easily evoked at intertrial intervals as short as 10 s without habituation. The high repetition rate allows a rapid determination of the threshold current intensities or the brain stimuli required to suppress the hindlimb withdrawal. The test is objective, avoids skin damage and sensitization and can be performed semi-automatically when implemented with a small PC.