Prunus host range of Plum pox virus (PPV) in the United States by aphid and graft inoculation
Prunus host range of Plum pox virus (PPV) in the United States by aphid and graft inoculation
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DOI：
10.1094/pd-91-0018
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Gildow, F. E.
中科院分区：
文献类型：
--
作者：
Damsteegt, V. D.;Scorza, R.;Gildow, F. E.
梅痘病是由梅痘病毒引起的一种严重的核果病毒性病害。为了确定哪些物种可以作为潜在宿主和病毒宿主，我们采用蚜虫传播和芽或片嫁接的方法评估了美国宾夕法尼亚州商品、观赏和野生李种对PPV分离株的敏感性。接种后，观察试验树的症状，用酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）分析，对健康桃子进行回测，并在4年内进行至少4次冷致休眠（CID）循环。33个李树种和栽培品种中有31个在蚜虫传播后全身感染。尽管多次尝试接种蚜虫，但在酸樱桃（P. cerasus）和雪泉（P. x 'Snofozam'）中未检测到全身感染。嫁接ppv侵染的芽材后，40个种和品种均发生侵染，但不同种对ppv的易感程度不同。在大多数物种中，尽管反复接种，一些单株植物在整个研究过程中仍保持PPV阴性。有些物种只能通过定量反转录(RT)-PCR检测感染情况。多数菌种症状明显，ELISA和RT-PCR检测均呈高阳性，CID后可反接种桃苗。我们的结果表明，广泛的本地和观赏李种对美国分离株PPV-D敏感。
Plum pox (Sharka) is a serious virus disease of stone fruits caused by the Plum pox virus (PPV). To determine which species could function as potential hosts and virus reservoirs, we used aphid transmission and bud or chip grafting to evaluate the susceptibility of commercial, ornamental, and wild Prunus species to isolates of PPV found in Pennsylvania, USA. Following inoculation, test trees were observed for symptoms, analyzed by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), back-assayed to healthy peach, and followed through at least four cold-induced dormancy (CID) cycles over 4 years. Thirty-one of 33 Prunus species and cultivars were systemically infected following aphid transmission. Systemic infection could not be detected in P. cerasus (sour cherry) and P. x 'Snofozam' (Snow Fountains) despite repeated aphid inoculation attempts. Following grafting of PPV-infected budwood, all 40 species and varieties became infected, although species differed in their susceptibility. Within most species, some individual plants remained PPV negative throughout the study despite repeated inoculations. Infection in some species could be detected only through quantitative reverse transcription (RT)-PCR. Most species displayed clear symptoms, were highly positive by ELISA and RT-PCR, and could be back-inoculated into peach seedlings following CID. Our results indicate that a wide range of native and ornamental Prunus species are susceptible to U.S. isolates of PPV-D.