Prevalence of duodenal ulcer-promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer in North Indian population
Prevalence of duodenal ulcer-promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer in North Indian population
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DOI：
10.1111/j.1523-5378.2007.00557.x
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发表时间：
2007-12-01
期刊：
影响因子：
4.4
通讯作者：
Ahuja, Vineet
中科院分区：
文献类型：
--
作者：
Arachchi, H. S. Jayasinghe;Kalra, Vijay;Ahuja, Vineet
背景资料：幽门螺杆菌（Helicobacter pylori，Hp）的十二指肠溃疡（DU）促进基因（dupA）是一种新的与DU发病风险相关的毒力标志物。采用聚合酶链反应（PCR）和杂交分析研究了北印度人群DU和功能性消化不良患者中存在的幽门螺杆菌。材料与方法：166例患者（DU 96例，功能性消化不良70例）纳入本研究。此外，还对H.通过用合适的引物对两条链上的PCR产物jhp 0917和jhp 0918进行测序来分析在这些患者中发现的pylori。结果：PCR和杂交分析表明，dupA基因在37.5%（36/ 96）的H.幽门螺杆菌菌株分离自DU患者和22.86%（16/ 70）的功能性消化不良患者（p =.05）。其中DU组35例（97.2%），功能性消化不良组14例（81.25%）。pylori cagA基因型阳性此外，dupA的存在与cagA阳性基因型显著相关（p = 0.02）。结论：dupA基因与DU的发生有明显的相关性。dupA基因可作为DU在该人群中的一个新的毒力标记。
Background: The duodenal ulcer (DU)-promoting gene (dupA) of Helicobacter pylori has been identified as a novel virulent marker associated with an increased risk for DU. The presence or absence of dupA gene of H. pylori present in patients with DU and functional dyspepsia in North Indian population was studied by polymerase chain reaction (PCR) and hybridization analysis. Materials and Methods: One hundred and sixty-six patients (96 DU and 70 functional dyspepsia) were included in this study. In addition, sequence diversity of dupA gene of H. pylori found in these patients was analyzed by sequencing the PCR products jhp0917 and jhp0918 on both strands with appropriate primers. Results: PCR and hybridization analyses indicated that dupA gene was present in 37.5% (36/ 96) of H. pylori strains isolated from DU patients and 22.86% (16/ 70) of functional dyspepsia patients (p =.05). Of these, 35 patients with DU (97.2%) and 14 patients with functional dyspepsia (81.25%) were infected by H. pylori positive for cagA genotype. Furthermore, the presence of dupA was significantly associated with the cagA-positive genotype (p =.02). Conclusion: Results of our study have shown that significant association of dupA gene with DU in this population. The dupA gene can be considered as a novel virulent marker for DU in this population.