Developmental potential and kinetics of pig embryos with different cytoplasmic volume
Developmental potential and kinetics of pig embryos with different cytoplasmic volume
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DOI：
10.1017/s0967199413000543
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发表时间：
2015-04-01
期刊：
影响因子：
1.7
通讯作者：
Callesen, Andhenrik
中科院分区：
文献类型：
--
作者：
Li, Juan;Li, Rong;Callesen, Andhenrik
研究了细胞质体积对猪胚胎体外发育的影响及其发育动力学。在手工克隆（HMC）过程中，根据相对于初始卵母细胞的大小，将选定的细胞质分为两组：相似于75%或相似于50%。在两个融合步骤和活化（第0天）之后，将重构的胚胎在体外培养6天。记录第2天的卵裂率以及第6天的囊胚率和细胞数。结果表明，在第一次融合时，相似50%与相似75%的细胞质的胚胎发育没有差异。该结果用于以下实验，其中测试了在第二次融合中改变细胞质体积以获得类似于75%至类似于200%的最终细胞质体积的效果。结果表明，当最终细胞质体积接近原始卵母细胞的75%时，获得的质量最低，而当最终细胞质体积接近原始卵母细胞的200%时，获得的质量最高。在用不同细胞质体积激活的孤雌生殖（PA）胚胎中观察到类似的结果。HMC和PA胚胎的发育动力学的一个共同模式被观察到：较小的组倾向于有较长的时间的前两个细胞周期，但随后的时间较短的形成桑椹胚和囊胚。总之，在体外生产的胚胎发育动力学的影响，由初始卵母细胞的细胞质体积，这进一步解释了重构胚胎的发育能力。
The effects of cytoplasmic volumes on development and developmental kinetics of in vitro produced porcine embryos were investigated. During hand-made cloning (HMC), selected cytoplasts were separated into two groups according to their size in relation to the initial oocyte: similar to 75% or similar to 50%. Following two fusion steps and activation (day 0), reconstructed embryos were cultured in vitro for 6 days. Cleavage rates on day 2 as well as blastocyst rates and cell numbers on day 6 were recorded. Results showed that embryo development was no different for similar to 50% versus similar to 75% cytoplasm at first fusion. This result was used in the following experiments, where the effect of varying cytoplasm volume in second fusion to obtain a final cytoplasm volume of similar to 75% to similar to 200% was tested. The results showed that the lowest quality was obtained when the final cytoplasm volume was similar to 75% and the highest quality at similar to 200% of the original oocyte. Similar results were observed in parthenogenetic (PA) embryos activated with different cytoplasmic volumes. A common pattern for the developmental kinetics of HMCand PA embryos was observed: the smaller group tended to have a longer time for the first two cell cycles, but subsequently a shorter time to form morula and blastocyst. In conclusion, the developmental kinetics of in vitro produced embryos was affected by the cytoplasm volume of the initial oocyte, and this further accounted for the developmental ability of the reconstructed embryos.