Characterization of a novel neuropathic pain model in mice
Characterization of a novel neuropathic pain model in mice
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DOI：
10.1097/wnr.0b013e328300ee0a
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发表时间：
2008-05-28
期刊：
影响因子：
1.7
通讯作者：
Donaldson, Lucy F.
中科院分区：
文献类型：
--
作者：
Hulse, Richard;Wynick, David;Donaldson, Lucy F.
我们描述了一个部分隐神经损伤（PSNI）模型的神经病理性疼痛的小鼠的特点。PSNI在小鼠中导致显著的机械异常性疼痛，而对温度刺激没有行为变化。PSNI还导致同侧爪腹屈，减少背侧后爪的功能性神经支配，并增加神经肽甘丙肽在背根神经节中的表达。我们已经使用PSNI模型来研究受损的初级传入神经元的电生理特性，证明可以识别单个纤维并研究其特性。在甘丙肽敲除小鼠中，PSNI未能诱导异常性疼痛，如先前在其他神经性疼痛模型中所报道的。PSNI可用于同时研究野生型和转基因小鼠的行为和神经生理变化。
We describe the characterization of a partial saphenous;nerve injury (PSNI) model of neuropathic pain in the mouse. PSNI resulted in significant mechanical allodynia in mice with no behavioural change to temperature stimulation. PSNI also resulted in ipsilateral paw ventroflexion, reduced functional innervation of the dorsal hindpaw and increased expression;in the dorsal root ganglion of the neuropeptide galanin. We have used the PSNI model to study the electrophysiological properties of injured primary afferent neurones, demonstrating that single fibres can be identified and their properties studied. In galanin knockout mice, PSNI failed to induce allodynia as previously reported in other neuropathic pain models. PSNI can be used to simultaneously study behavioural and neurophysiological changes in wild-type and transgenic mice.