Purification and characterization of plant dynamin from tobacco BY-2 cells.
Purification and characterization of plant dynamin from tobacco BY-2 cells.
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DOI：
10.1093/pcp/pcj086
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发表时间：
2006-08
影响因子：
4.9
通讯作者：
T. Hamada;H. Igarashi;Maki Yao;T. Hashimoto;T. Shimmen;S. Sonobe
中科院分区：
文献类型：
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作者：
T. Hamada;H. Igarashi;Maki Yao;T. Hashimoto;T. Shimmen;S. Sonobe
我们从烟草BY-2培养细胞的MAP（微管相关蛋白）组分中纯化了一种84 kDa的多肽。LC/MS/MS（液相色谱-串联质谱法）分析显示该多肽是AtDRP 3（拟南芥动力蛋白相关蛋白3）的烟草同系物。电子显微镜显示NtDRP 3（烟草动力蛋白相关蛋白3）组装形成丝状结构。当GDP加入到NtDRP 3级分中时，细丝消失，出现许多颗粒。生化分析表明，NtDRP 3可以绑定和捆绑微管和肌动蛋白丝在体外。
We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro.