Effect of antipsychotic drugs and selective dopaminergic antagonists on dopamine-induced facilitatory activity in prelimbic cortical pyramidal neurons.: An in vitro study
Effect of antipsychotic drugs and selective dopaminergic antagonists on dopamine-induced facilitatory activity in prelimbic cortical pyramidal neurons.: An in vitro study
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DOI：
10.1016/s0306-4522(99)00123-2
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发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Borsini, F
中科院分区：
文献类型：
--
作者：
Ceci, A;Brambilla, A;Borsini, F
细胞内记录来自119个锥体神经元位于前边缘皮层，5个在背扣带皮层，1个在边缘下皮层，1个在边缘前和扣带皮层的边界和2个在边缘前和边缘下皮层的边界。这些锥体神经元的被动膜特性（即静息膜电位，输入膜电阻，河豚毒素敏感动作电位的形状，尖峰频率适应与一个突出的postspike后超极化，河豚毒素敏感的内向整流在去极化方向和爆裂的情况下）表明，他们类似于定期尖峰或内在爆裂锥体神经元。浴应用多巴胺（EC 50为1.8 μ M）产生了可逆的易化作用，对所有119个锥体神经元位于中间层的前边缘皮层。在多巴胺的应用过程中，没有检测到膜电位的一致变化。多巴胺对9个不位于边缘前皮质的锥体神经元没有影响。氟哌啶醇、利培酮、奎替利、氯氮平和选择性D-4多巴胺能受体拮抗剂L-745，870可浓度依赖性地拮抗多巴胺在前边缘皮层的易化作用，而选择性D_2/D_3多巴胺能受体拮抗剂（-）-舒必利则不拮抗。（+）-SCH 23390是一种选择性D-1/D-5多巴胺受体拮抗剂，与多巴胺类似，其本身产生易化效应，当与多巴胺共同给药时产生累加效应，这些结果提供了证据，表明多巴胺对位于前边缘皮层中层的锥体神经元具有特异性易化效应。抗精神病药物和L-745，870阻断多巴胺的这种作用。(C)1999年IBRO。出版社：Elsevier Science Ltd
Intracellular recordings were obtained from 119 pyramidal neurons localized in prelimbic cortex, five in the dorsal cingulate cortex, one in the infralimbic cortex, one in the border of prelimbic and cingulate cortex and two in the border of prelimbic and infralimbic cortex. The passive membrane properties of these pyramidal neurons (i.e. resting membrane potential, input membrane resistance, shape of the tetrodotoxin-sensitive action potentials, spike frequency adaptation with a prominent postspike afterhyperpolarization, tetrodotoxin-sensitive inward rectification in the depolarizing direction and the absence of bursting) suggested that they resembled regular spiking or intrinsically bursting pyramidal neurons. Bath application of dopamine (EC50 of 1.8 mu M) produced a reversible facilitatory effect on all 119 pyramidal neurons localized in the middle layer of the prelimbic cortex. No consistent change in membrane potential was detected during the application of dopamine. No effect of dopamine was noted on the nine pyramidal neurons that were not localized in the prelimbic cortex. The facilitatory effect of dopamine in prelimbic cortex was concentration dependently antagonized by haloperidol, risperidone, quetiapine, clozapine and by the selective D-4 dopaminergic receptor antagonist L-745,870, but not by the selective D2/D3 dopaminergic receptor antagonist (-)-sulpiride. (+)-SCH 23390, which is a selective D-1/D-5 dopamine receptor antagonist, produced, similarly to dopamine, a facilitatory effect per se, and an additive effect when co-administered with dopamine.These results provide evidence that dopamine has a facilitatory effect specifically on pyramidal neurons localized in the middle layer of prelimbic cortex. Antipsychotic drugs and L-745,870 block this effect of dopamine. (C) 1999 IBRO. Published by Elsevier Science Ltd.