Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer
Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer
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DOI：
10.1007/s00604-012-0932-0
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发表时间：
2013-02-01
影响因子：
5.7
通讯作者：
Kuncova, Gabriela
中科院分区：
文献类型：
--
作者：
Pospiskova, Kristyna;Safarik, Ivo;Kuncova, Gabriela
我们已经开发了一种光纤生物传感器与纳入磁性微粒生物胺的测定。将豌豆二胺氧化酶固定在壳聚糖包被的磁性微粒上或用铁磁流体修饰的商业微珠上。固定化酶和钌络合物都被掺入到UV固化的无机-有机聚合物复合材料中，并沉积在通过光纤连接到电光检测器的透镜上。这种酶在消耗氧气的情况下催化胺的氧化。后者通过测量钌络合物的猝灭荧光寿命来确定。生物胺腐胺和尸胺的检测限为25-30 μ mol L-1，响应线性高达1 mmol L-1的浓度。
We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic-organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25-30 mu mol L-1, and responses are linear up to a concentration of 1 mmol L-1.