Time-course proteomics dataset monitoring HeLa cells subjected to DTT induced endoplasmic reticulum stress.
Time-course proteomics dataset monitoring HeLa cells subjected to DTT induced endoplasmic reticulum stress.
复制标题
时程蛋白质组学数据集监测 HeLa 细胞受到 DTT 诱导的内质网应激的影响。
DOI：
10.1016/j.dib.2016.07.038
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发表时间：
2016
期刊：
影响因子：
1.2
通讯作者：
Vogel,Christine
中科院分区：
文献类型：
--
作者：
Cheng,Zhe;Rendleman,Justin;Vogel,Christine
本文所述的数据提供了HeLa细胞蛋白质组对二硫苏糖醇（DTT）诱导内质网（ER）应激的动态响应分析。在内质网应激期间，错误折叠和未折叠蛋白质在内质网腔中的积累启动未折叠蛋白质反应（UPR），导致蛋白质的大规模重新分布。我们使用无标记质谱法监测HeLa细胞在30小时的时间过程中的蛋白质组学变化，监测8个时间点（0，0.5，1，2，8，16，24和30小时）。这些数据与研究文章“Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress”[1]相关，该文章讨论了1237种蛋白质的核心数据集。在这里，我们提供了2131种蛋白质的扩展数据集。原始质谱数据和分析结果已通过PRIDE合作伙伴存储库（数据集标识符为PRIDE：PXD 002039）保存到ProteomeXchange Consortium（proteomecentral.proteomexchange.org）。
The data described here provide an analysis of the dynamic response of HeLa cell proteome to dithiothreitol (DTT) inducing stress of the endoplasmic reticulum (ER). During ER stress, accumulation of misfolded and unfolded proteins in the lumen of the ER initiates the Unfolded Protein Response (UPR), resulting in a large-scale redistribution of proteins. We used label-free mass spectrometry to monitor the proteomic changes of HeLa cells during a 30-h time course, monitoring eight time points (0, 0.5, 1, 2, 8, 16, 24, and 30 h). The data are associated with the research article “Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress” [1], which discusses a core dataset of 1237 proteins. Here, we present the extended dataset of 2131 proteins. The raw mass spectrometry data and the analysis results have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PRIDE: PXD002039.