“Quorum Non-Sensing”: Social Cheating and Deception in Vibrio cholerae
“Quorum Non-Sensing”: Social Cheating and Deception in Vibrio cholerae
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DOI：
10.1128/aem.00586-15
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发表时间：
2015-03
影响因子：
4.4
通讯作者：
D. Katzianer;Hui Wang;R. Carey;Jun Zhu
中科院分区：
文献类型：
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作者：
D. Katzianer;Hui Wang;R. Carey;Jun Zhu
摘要群体感应（Quorum sensing，QS）是细菌广泛利用的一种调节行为的机制。在霍乱弧菌中，这一过程对环境生存和致病性很重要，但有趣的是，很大比例的天然分离株是QS缺陷的。在这里，我们表明，QS缺陷型突变体可以传播的社会骗子停止生产的胞外蛋白酶的条件下，需要他们的增长。我们进一步表明，突变体刺激生物膜的形成，并过度代表生物膜相比，反硝化社区，在此基础上，我们认为，QS缺陷型突变体可能有副作用，提高自然种群的环境耐受性，由于生物膜的固有抗性特性。有趣的是，尽管突变体不能对这些诱导剂做出反应，但高频率的QS缺陷个体并不影响QS信号分子的产生，这表明这些变体在需要QS的条件下通过假信号积极欺骗。综上所述，我们的研究结果表明，社会欺骗可能会导致霍乱弧菌自然种群中出现QS缺乏症。
ABSTRACT Quorum sensing (QS) is widely used by bacteria to coordinate behavior in response to external stimuli. In Vibrio cholerae, this process is important for environmental survival and pathogenesis, though, intriguingly, a large percentage of natural isolates are QS deficient. Here, we show that QS-deficient mutants can spread as social cheaters by ceasing production of extracellular proteases under conditions requiring their growth. We further show that mutants stimulate biofilm formation and are over-represented in biofilms compared to planktonic communities; on this basis, we suggest that QS-deficient mutants may have the side effect of enhancing environmental tolerance of natural populations due to the inherent resistance properties of biofilms. Interestingly, high frequencies of QS-deficient individuals did not impact production of QS signaling molecules despite mutants being unable to respond to these inducers, indicating that these variants actively cheat by false signaling under conditions requiring QS. Taken together, our results suggest that social cheating may drive QS deficiency emergence within V. cholerae natural populations.