miR156-Targeted SBP-Box Transcription Factors Interact with DWARF53 to Regulate TEOSINTE BRANCHED1 and BARREN STALK1 Expression in Bread Wheat
miR156-Targeted SBP-Box Transcription Factors Interact with DWARF53 to Regulate TEOSINTE BRANCHED1 and BARREN STALK1 Expression in Bread Wheat
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DOI：
10.1104/pp.17.00445
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发表时间：
2017-07-01
期刊：
影响因子：
7.4
通讯作者：
Sun, Jiaqiang
中科院分区：
文献类型：
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作者：
Liu, Jie;Cheng, Xiliu;Sun, Jiaqiang
遗传和环境因素影响面包小麦(Triticum Aestivum)的株型，进而决定籽粒产量。在这项研究中，我们证明了miR156控制面包小麦的植株构型。我们发现，在面包小麦品种科农199中过表达tae-miR156导致分蘖数增加和小穗形成的严重缺陷，这可能是由于tae-miR156介导的一组鳞状启动子结合蛋白(SPL)基因的抑制。此外，我们还发现两个基因teosinte BRANCHED1(TaTB1)和barren STALK1(TabA1)在不同植物物种中起着保守的植物结构调控作用，但在tae-miR156-OE面包小麦中的表达显著降低。值得注意的是，我们证明了与转录辅阻遏子TOBLIST物理上相关的斯特里内酯(SL)信号抑制子DWARF53(TaD53)可以直接与miR156控制的TaSPL3/17的N末端结构域相互作用。最重要的是，在瞬时表达系统中，TaSPL3/17介导的TabA1和TaTB1的转录激活在很大程度上可以被TaD53抑制。我们的结果揭示了在面包小麦分蘖和小穗发育过程中miR156-TaSPLs和SL信号通路之间的潜在关联。
Genetic and environmental factors affect bread wheat (Triticum aestivum) plant architecture, which determines grain yield. In this study, we demonstrate that miR156 controls bread wheat plant architecture. We show that overexpression of tae-miR156 in bread wheat cultivar Kenong199 leads to increased tiller number and severe defects in spikelet formation, probably due to the tae-miR156-mediated repression of a group of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes. Furthermore, we found that the expression of two genes TEOSINTE BRANCHED1 (TaTB1) and BARREN STALK1 (TaBA1), whose orthologous genes in diverse plant species play conserved roles in regulating plant architecture, is markedly reduced in the tae-miR156-OE bread wheat plants. Significantly, we demonstrate that the strigolactone (SL) signaling repressor DWARF53 (TaD53), which physically associates with the transcriptional corepressor TOPLESS, can directly interact with the N-terminal domains of miR156-controlled TaSPL3/ 17. Most importantly, TaSPL3/ 17-mediated transcriptional activation of TaBA1 and TaTB1 can be largely repressed by TaD53 in the transient expression system. Our results reveal potential association between miR156-TaSPLs and SL signaling pathways during bread wheat tillering and spikelet development.