Identification of a 43-kDa outer membrane protein of Fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other Fusobacterium species
Identification of a 43-kDa outer membrane protein of Fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other Fusobacterium species
复制标题
鉴定坏死梭杆菌的 43 kDa 外膜蛋白，该蛋白与其他梭杆菌属的成孔蛋白具有相似性
DOI：
10.1016/j.rvsc.2013.01.016
复制
发表时间：
2013-08-01
影响因子：
2.4
通讯作者：
Guo, Donghua
中科院分区：
文献类型：
--
作者：
Sun, Dongbo;Zhang, Hong;Guo, Donghua
根据具核梭杆菌外膜蛋白（OMP）基因的核苷酸序列设计一对引物，扩增坏死梭杆菌OMP基因。进一步分析其分子特性和在梭杆菌属中的遗传特性。我们在F. necrophorum的成孔蛋白，经信号肽、AT富集区、跨膜区和保守基序分析表明，该蛋白与革兰氏阴性厌氧菌的其它成孔蛋白具有相同的性质。预测的43 K外膜蛋白与F.具核梭杆菌、变异梭杆菌、橄榄梭杆菌、牙周梭杆菌、死亡梭杆菌、产角梭杆菌和F. necrophorum（假想蛋白）。在45个梭杆菌外膜蛋白序列中发现了11个共同的保守结构域和10个共同的可变结构域。保守区和可变区的分布与预测的跨膜区、细胞表面暴露区和B细胞表位区高度相关。系统发育分析表明，F. necrophorum与F. nucleatum和F.牙周病这些数据将有助于加深对F.尸斑(c)2013爱思唯尔有限公司保留所有权利。
A pair of primers was designed in an attempt to amplify outer membrane protein (OMP) gene of Fusobacterium necrophorum based on nucleotide sequence of the OMP of Fusobacterium nucleatum. Further analysis was performed to characterize its molecular properties and phylogeny in the genus Fusobacterium. We identified a predicated 43 kDa outer membrane protein (43 K OMP) in F. necrophorum, which showed the same properties as other pore-forming proteins of Gram-negative anaerobic bacteria according to analysis of signal peptide, AT-rich, membrane-spanning region and conserved motifs. The predicated 43 K OMP exhibited 70.22%, 62.04%, 56.75%, 58.72%, 51.59%, 31.49% and 50.26% amino acid identity with the OMPs of F. nucleatum, Fusobacterium varium, Fusobacterium ucerans, Fusobacterium periodonticum, Fusobacterium mortiferum, Fusobacterium gonidiaformans and F. necrophorum (hypothetical protein), respectively. 11 common conserved domains and 10 common variable domains were found among the 45 aligned OMPs of Fusobacterium species. Distributions of the conserved and variable domains were highly associated with predicted membrane-spanning regions, cell surface exposed regions and B-cell epitope regions. Phylogenetic analysis revealed the predicated 43 K OMP of F. necrophorum was closely related with the OMPs from F. nucleatum and F. periodonticum. These data will increase understanding of pathogenesis and genetic evolution of F. necrophorum. (c) 2013 Elsevier Ltd. All rights reserved.