Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode
Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode
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DOI：
10.1016/j.aca.2006.07.052
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发表时间：
2006-11-24
影响因子：
6.2
通讯作者：
Palleschi, Giuseppe
中科院分区：
文献类型：
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作者：
Arduini, Fabiana;Ricci, Francesco;Palleschi, Giuseppe
在本文中，使用钴酞菁和普鲁士蓝改性丝网印刷电极进行了比较研究。这两种电极均表现出易于制备以及对硫代胆碱的高灵敏度（共酞菁和普鲁士蓝的 LOD 分别为 5 x 10(-7) 和 5 x 10(-6) M），在农药测量方面具有很高的潜力。然后选择普鲁士蓝修饰的丝网印刷电极用于连续的酶固定化，因为它们在之前的工作中表现出更高的操作稳定性。使用AChE和BChE酶，研究这两种酶对不同农药的抑制效果。基于 AChE 的生物传感器对涕灭威（50 ppb 抑制 50%）和西维因（85 ppb 抑制 50%）具有更高的灵敏度，而 BChE 生物传感器对对氧磷（4 ppb 抑制 50%）和毒死蜱-甲基氧磷（1 ppb 抑制 50%）具有更高的亲和力。还测试了实际样品以评估基质效应，并获得了 79% 至 123% 之间的回收率值。 (c) 2006 Elsevier B.V. 保留所有权利。
In the present paper, a comparative study using Co-phthalocyanine and Prussian Blue-modified screen-printed electrodes, has been performed. Both the electrodes have demonstrated an easiness of preparation together with high sensitivity towards thicoholine (LOD= 5 x 10(-7) and 5 x 10(-6) M for Co-phthalocyanine and Prussian Blue, respectively) with high potentialities for pesticide measurement. Prussian Blue-modified screen-printed electrodes were then selected for successive enzyme immobilization due to their higher operative stability demonstrated in previous works. AChE and BChE enzymes were used and inhibition effect of different pesticides was studied with both the enzymes. AChE-based biosensors have demonstrated a higher sensitivity towards aldicarb (50% inhibition with 50 ppb) and carbaryl (50% inhibition with 85 ppb) while BChE biosensors have shown a higher affinity towards paraoxon (50% inhibition with 4 ppb) and chlorpyrifos-methyl oxon (50% inhibition with I ppb). Real samples were also tested in order to evaluate the matrix effect and recovery values comprised between 79 and 123% were obtained. (c) 2006 Elsevier B.V. All rights reserved.