Oral treatment with Lactobacillus reuteri attenuates depressive-like behaviors and serotonin metabolism alterations induced by chronic social defeat stress.
Oral treatment with Lactobacillus reuteri attenuates depressive-like behaviors and serotonin metabolism alterations induced by chronic social defeat stress.
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DOI：
10.1016/j.jpsychires.2019.12.013
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发表时间：
2019-12
影响因子：
4.8
通讯作者：
Ruining Xie;Pei Jiang;Li Lin;Jian Jiang;Bin Yu;Jingjing Rao;Hui Liu;Wei Wei-Wei;Y. Qiao
中科院分区：
文献类型：
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作者：
Ruining Xie;Pei Jiang;Li Lin;Jian Jiang;Bin Yu;Jingjing Rao;Hui Liu;Wei Wei-Wei;Y. Qiao
背景肠-脑双向相互作用的改变被认为参与了神经精神疾病的发病机制。考虑到肠道微生物群，神经功能和行为之间的假定联系，本研究调查了微生物诱导的肠道到大脑的信号传导的潜力，以调节压力对抑郁样行为和5-羟色胺代谢的影响。reuteri 3）或赋形剂28天，并评估行为和5-羟色胺代谢的改变。采用16 S rRNA序列分析和气相色谱分析方法对肠道菌群和短链脂肪酸进行分析。reuteri 3可改善抑郁样行为，抑制梭菌和阿氏杆菌相对丰度的增加，改善应激引起的乳杆菌、异芽孢杆菌和Sutterella相对丰度的下降，显著增加双歧杆菌的比例。reuteri 3可降低抑郁症组的醋酸盐和总SCFAs水平。抑郁样小鼠血液和结肠5-HT含量降低，但L. reuteri 3治疗。此外，L. reuteri 3给药增加了参与5-羟色胺生物合成的酶的表达，但抑制了参与色氨酸代谢的酶的表达，沿着结肠和前额皮质中的犬尿氨酸途径。reuteri 3可以防止由慢性社会失败压力引起的抑郁样行为。L. reuteri 3与改善肠道微生物群和5-羟色胺代谢有关。
BackgroundAlterations in bidirectional gut–brain interactions are believed to be involved in the pathogenesis of neuropsychiatric diseases. Considering the putative connections among gut microbiota, neural function, and behavior, this study investigated the potential of microbe-induced gut–to–brain signaling to modulate the impact of stress on depressive-like behaviors and serotonin metabolism.MethodsDepression-susceptible mice induced by chronic social defeat stress received oral treatment of eitherLactobacillusreuteri3 (L. reuteri3) or vehicle for 28 days, and alterations in behavior and serotonin metabolism were assessed. 16S rRNA sequencing and gas chromatograph were employed to analyze the gut microbiota community and short-chain fatty acids (SCFAs).ResultsTreatment withL. reuteri3 ameliorated depressive-like behaviors, suppressed the increase in the relative abundances ofClostridialesandAdlercreutzia, improved the decrease in abundances ofLactobacillus,Allobaculum, andSutterellainduced by stress, and significantly increased the proportion ofBifidobacterium.L. reuteri3 reduced the acetate and total SCFAs levels in the depression group. Blood and colon 5-HT were decreased in depressive-like mice but were significantly ameliorated afterL. reuteri3 treatment. Moreover,L. reuteri3 administration increased the expression of enzymes involved in serotonin biosynthesis but suppressed that of the enzymes involved in tryptophan metabolism along the kynurenine pathway in colon and prefrontal cortex.ConclusionsDespite the complexity of the gut microbiota, exposure to a single microbial strainL. reuteri3 can protect against depressive-like behaviors induced by chronic social defeat stress. The anti-depressive effects ofL. reuteri3 were associated with improved gut microbiota and serotonin metabolism.