Determination of tannic acid after precipitation with bovine serum albumin by visible light scattering in a flow injection system
Determination of tannic acid after precipitation with bovine serum albumin by visible light scattering in a flow injection system
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DOI：
10.1007/s00604-010-0301-9
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发表时间：
2010-03
影响因子：
5.7
通讯作者：
T. Cheng;Hsien-Yi Hsiao;Chien-Yu Chung;Po-Chung Chen;Richie L. C. Chen
中科院分区：
文献类型：
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作者：
T. Cheng;Hsien-Yi Hsiao;Chien-Yu Chung;Po-Chung Chen;Richie L. C. Chen
单宁酸可以与牛血清白蛋白（BSA）形成沉淀，并利用流动注射系统和600 nm光散射检测对其进行了研究。用粒度法对光散射结果进行了验证。与BSA在pH <4或bbbb8的溶液中产生的背景相比，散射信号可以忽略不计。通过使用十二烷基硫酸钠洗涤液，可以有效地去除流动池管道壁上的任何沉淀物。单宁酸的分析范围为70 ~ 250µgml -1。结果表明，增加牛血清白蛋白浓度可提高灵敏度和分析范围。该系统可用于pH值在5到7之间的溶液。结果与酒石酸亚铁法分析单一单宁酸和两种茶单宁酸的结果具有密切的相关性（r2> 0.97）。
Tannic acid can form a precipitate with bovine serum albumin (BSA), and this was explored for its determination using a flow injection system along with detection via light scattering at 600 nm. The results on light scattering are confirmed using a particle sizing method. Scattering signals are negligible compared to the background generated by BSA in solutions with a pH of <4 or >8. Any precipitates on the wall of the flow cell pipeline were effectively removed by using a sodium dodecyl sulfate washing solution. The analytical range is from 70 to 250 µgmL-1of tannic acid. The results imply that increasing BSA concentrations will enhance sensitivity and analytical ranges. The system can be used in solutions of pH values between 5 and 7. The results correlated closely (r2> 0.97) with those obtained by the ferrous tartrate method when analyzing a single tannic acid and two tea tannins.