Salicylic acid interferes with clathrin-mediated endocytic protein trafficking
Salicylic acid interferes with clathrin-mediated endocytic protein trafficking
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DOI：
10.1073/pnas.1220205110
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发表时间：
2013-05-07
影响因子：
11.1
通讯作者：
Friml, Jiri
中科院分区：
文献类型：
--
作者：
Du, Yunlong;Tejos, Ricardo;Friml, Jiri
通过胞吞作用从细胞表面清除物质是调节质膜（PM）驻留蛋白（如受体或转运蛋白）活性的有效机制。水杨酸（SA）是一种重要的植物激素，传统上与病原体防御有关。在这里，我们描述了SA对蛋白质亚细胞内吞循环的意想不到的影响。外源性处理和内源性增强SA水平抑制不同PM蛋白的内吞作用。SA对内吞作用的影响不涉及转录或已知的SA信号通路的转录调节组分。SA可能针对涉及外壳蛋白网格蛋白的内吞机制，因为SA干扰在PM和网格蛋白缺陷突变体的网格蛋白发病率不太敏感SA对生长素分布和根弯曲的影响，在向重力反应。相比之下，SA不影响病原体反应期间鞭毛蛋白传感2（FLS2）受体的配体诱导的内吞作用。我们的数据表明，建立SA的影响转录在植物免疫和非转录的影响SA网格蛋白介导的内吞作用是独立的机制，SA调节植物生理的不同方面。
Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology.