Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback.
Enrichment of Burkholderia in the Rhizosphere by Autotoxic Ginsenosides to Alleviate Negative Plant-Soil Feedback.
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DOI：
10.1128/spectrum.01400-21
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发表时间：
2021-12-22
影响因子：
3.7
通讯作者：
Yang M
中科院分区：
文献类型：
--
作者：
Luo L;Wang L;Deng L;Mei X;Liu Y;Huang H;Du F;Zhu S;Yang M
土壤中自身毒素和土传病原菌的积累是植物-土壤负反馈(NPSF)的主要驱动因素。人们一致认为，植物可以通过改变根际微生物群来增强其对生物或非生物胁迫的适应能力。然而，目前尚不清楚自体毒素是否可以丰富微生物以降解自己或拮抗土壤传播的病原体。在这里，我们发现该微生物群降解三七根际土壤中的三七根际土壤中的自毒人参皂苷，属于三萜苷类，并具有拮抗病原菌的作用。16S rRNA测序分析表明，根际土壤中的细菌群落发生了明显的变化，鉴定出根际土壤中主要的富含人参皂苷的细菌为伯克霍尔德氏菌-卡巴列尼亚-副伯克霍尔德氏菌(BCP)。分离到8株属于BCP群的菌株，其中伯克霍尔德氏菌B36菌株表现出同时降解自毒人参皂苷(Rb1、Rg1和Rd)和拮抗土传病原菌破坏伊藤的能力。有趣的是，人参皂苷能够刺激B36的生长和生物膜的形成，最终提高B36对I.destructans的拮抗能力和在根际土壤中的定植能力。综上所述，三七分泌的自毒人参皂苷可以丰富根际有益微生物，同时降解自毒物质和拮抗病原菌，为缓解NPSF提供了一种新的生态策略。重要的自毒人参皂苷由三七分泌到土壤中，可以丰富伯克霍尔德氏菌。通过降解自身毒素和拮抗根腐病病原菌来缓解植物土壤负反馈(NPSF)。人参皂苷对伯克霍尔德氏菌的生长和生物膜形成有促进作用。B36，最终提高了伯克霍尔德氏菌的拮抗能力。B36对一种土传病原菌的侵染及其在土壤中的定殖。这一生态策略可以通过控制根际微生物群同时降解自体毒素和拮抗病原菌来缓解NPSF。
The accumulation of autotoxins and soilborne pathogens in soil was shown to be the primary driver of negative plant-soil feedback (NPSF). There is a concerted understanding that plants could enhance their adaptability to biotic or abiotic stress by modifying the rhizosphere microbiome. However, it is not clear whether autotoxins could enrich microbes to degrade themselves or antagonize soilborne pathogens. Here, we found that the microbiome degraded autotoxic ginsenosides, belonging to triterpenoid glycosides, and antagonized pathogens in the rhizosphere soil of Panax notoginseng (sanqi). Deep analysis by 16S rRNA sequencing showed that the bacterial community was obviously changed in the rhizosphere soil and identified the Burkholderia-Caballeronia-Paraburkholderia (BCP) group as the main ginsenoside-enriched bacteria in the rhizosphere soil. Eight strains belonging to the BCP group were isolated, and Burkholderia isolate B36 showed a high ability to simultaneously degrade autotoxic ginsenosides (Rb1, Rg1, and Rd) and antagonize the soilborne pathogen Ilyonectria destructans. Interestingly, ginsenosides could stimulate the growth and biofilm formation of B36, eventually enhancing the antagonistic ability of B36 to I. destructans and the colonization ability in the rhizosphere soil. In summary, autotoxic ginsenosides secreted by P. notoginseng could enrich beneficial microbes in the rhizosphere to simultaneously degrade autotoxins and antagonize pathogen, providing a novel ecological strategy to alleviate NPSF. IMPORTANCE Autotoxic ginsenosides, secreted by sanqi into soil, could enrich Burkholderia sp. to alleviate negative plant-soil feedback (NPSF) by degrading autotoxins and antagonizing the root rot pathogen. In detail, ginsenosides could stimulate the growth and biofilm formation of Burkholderia sp. B36, eventually enhancing the antagonistic ability of Burkholderia sp. B36 to a soilborne pathogen and the colonization of B36 in soil. This ecological strategy could alleviate NPSF by manipulating the rhizosphere microbiome to simultaneously degrade autotoxins and antagonize pathogen.