Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers
Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers
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DOI：
10.1021/cm071451m
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发表时间：
2007-11-13
影响因子：
8.6
通讯作者：
Kataoka, Kazunori
中科院分区：
文献类型：
--
作者：
Li, Yuan;Jang, Woo-Dong;Kataoka, Kazunori
研究了一系列聚苄醚类树枝状卟啉（Gn = n代树枝状大分子，n = 1-3）作为光动力学治疗（PDT）光敏剂的应用。聚乙二醇-聚赖氨酸嵌段共聚物（PEG-β-PLL）与DP之间的聚离子复合物（PIC）是通过带正电荷的聚赖氨酸（PLL）片段与带负电荷的DP外围之间的静电相互作用形成的。动态光散射（DLS）测量和透射电子显微镜。(TEM)结果表明，G3形成了核-壳型纳米载体胶束，而G1和G2形成了具有相对高的多分散性的不规则形状的纳米颗粒。DP负载的PIC纳米载体的电子物理性质强烈依赖于DP的产生。在GI和G2的情况下，它们的荧光寿命和氧消耗能力显著降低的PIC纳米载体的形成，而G34负载的PIC纳米载体表现出几乎相当的荧光寿命和氧消耗能力的自由G3。将DP掺入PIC纳米载体中导致细胞摄取的明显增加，但与生成呈负相关。或者，纳米载体内的DP的光细胞毒性随着生成的增加而增加，尽管细胞摄取减少。通过将摄取量的影响与光细胞毒性相关联，PIC纳米载体显示出依赖于DP的产生的PDT功效的显著增强。
A series of poly(benzyl ether) dendrimer porphyrins (DPs) (Gn = n-generation dendrimer, n = 1-3) was examined as potential photosensitizers for photodynamic therapy (PDT). Polyion complexes (PICs) between the DPs and poly(ethylene glycol)-block-poly(L-lysine) (PEG-beta-PLL) were formed via an electrostatic interaction between the positively charged poly(L-lysine) (PLL) segment and negatively charged periphery of the DPs. Dynamic light scattering (DLS) measurements and transmission electron microscopy. (TEM) showed that G3 formed a core-shell-type nanocarrier micelle, whereas G1 and G2 formed irregular-shaped nanoparticles with a relatively high polydispersity. The photophysical properties of the DP-loaded PIC nanocarriers strongly depend on the generation of the DPs. In the case of GI and G2, their fluorescence lifetime and oxygen consumption ability were significantly reduced by the formation of the PIC nanocarriers, whereas the G34oaded PIC nanocarrier exhibited almost comparable fluorescence lifetimes and oxygen consumption abilities to the free G3. The incorporation of DPs into PIC nanocarriers resulted in an appreciable increase in the cellular uptake, yet inversely correlated with the generation. Alternatively, the photocytotoxicity of the DPs within the nanocarriers increased with an increase in the generation despite a decrease in the cellular uptake. By correlating the effects of the uptake amount with the photocytotoxicity, the PIC nanocarriers showed remarkable enhancement of the PDT efficacy dependent on the generation of DPs.