Acrosome reaction in sperm of the toad, bufo bufo japonicus
Acrosome reaction in sperm of the toad, bufo bufo japonicus
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蟾蜍精子顶体反应
DOI：
10.1002/mrd.1120060406
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
C. Katagiri
中科院分区：
文献类型：
--
作者：
N. Yoshizaki;C. Katagiri
蟾蜍精子的顶体由顶体帽和纤维状穿孔器组成。当精子与输卵管直部提取物（PRE）孵育30-60分钟时，顶体外膜与上覆质膜在几个点处融合，伴随着顶体帽内容物的损失。因此暴露的顶体内膜与顶体区尾端的质膜融合。大豆胰蛋白酶抑制剂可完全抑制PRE诱导的顶体反应。在受精卵的最内层胶状层中发现的精子具有完整的顶体，但那些穿过卵黄被或定位在卵黄周隙中的精子与PRE处理时的顶体反应方式相同。这些观察结果，结合最近的证据表明，参与受精的直部物质，表明顶体反应发生在一个精子或接近表面的卵黄外套是一个反应的物质，这是来自直部和沉积在卵黄外套。
The acrosome in the sperm of the toad, Bufo bufo japonicus, consists of a membrane-limited acrosomal cap and a fibrous perforatorium. When sperm are incubated with the oviducal pars recta extract (PRE) for 30–60 min, the outer acrosomal membrane fuses with the overlying plasma membrane at several points with concomitant loss of the contents of the acrosomal cap. The inner acrosomal membrane thus exposed fuses with the plasma membrane at the caudal end of the acrosomal region. This PRE-induced acrosome reaction is completely inhibited by soybean trypsin inhibitor. Sperm found in the innermost jelly layer of inseminated eggs possess an intact acrosome, but those either passing through the vitelline coat or localizing in the perivitelline space are acrosome-reacted in the same manner as when treated with PRE. These observations, combined with recent evidence showing involvement of the pars recta substance in fertilization, indicate that the acrosome reaction occurring in a fertilizing sperm at or near the surface of the vitelline coat is a response to a substance that is derived from the pars recta and deposited in the vitelline coat.