Teaching an Old Virus New Tricks: A Review on New Approaches to Study Age-Old Questions in Influenza Biology.
Teaching an Old Virus New Tricks: A Review on New Approaches to Study Age-Old Questions in Influenza Biology.
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DOI：
10.1016/j.jmb.2019.04.038
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发表时间：
2019-10
影响因子：
5.6
通讯作者：
S. Lakdawala;Nara Lee;C. Brooke
中科院分区：
文献类型：
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作者：
S. Lakdawala;Nara Lee;C. Brooke
流感病毒已经被研究了超过80 年，但关于病毒基本生命周期的很多信息仍然未知。然而，新的成像，生化和测序技术已经揭示了许多古老的流感病毒生物学问题的重要见解。在这篇综述中，我们将涵盖成像技术的作用，以描述流感病毒组装的独特方面，生化技术来研究病毒基因组组织，以及下一代测序来探索流感基因组进化。我们的目标是简要概述如何使用新兴技术来回答有关流感病毒的基本问题。这不是一个新兴技术的全面列表，而是我们认为将继续对流感生物学领域做出重大贡献的技术。
Influenza viruses have been studied for over 80 years, yet much about the basic viral lifecycle remain unknown. However, new imaging, biochemical, and sequencing techniques have revealed significant insight into many age-old questions of influenza virus biology. In this review, we will cover the role of imaging techniques to describe unique aspects of influenza virus assembly, biochemical techniques to study viral genomic organization, and next-generation sequencing to explore influenza genomic evolution. Our goal is to provide a brief overview of how emerging techniques are being used to answer basic questions about influenza viruses. This is not a comprehensive list of emerging techniques, rather ones that we feel will continue to make significant contributions to field of influenza biology.