Preparation, immunological characterization and polyclonal antibody development for recombinant epitope tandem derived from bovine -lactoglobulin
Preparation, immunological characterization and polyclonal antibody development for recombinant epitope tandem derived from bovine -lactoglobulin
复制标题
DOI：
10.1080/09540105.2016.1183596
复制
发表时间：
2016-11-01
影响因子：
3
通讯作者：
Chen, Hongbing
中科院分区：
文献类型：
--
作者：
He, Shengfa;Li, Xin;Chen, Hongbing
牛乳球蛋白(BLG)是牛奶中的主要过敏原之一。表位在食物过敏中起着关键作用，而大多数已开发的抗体并不能识别与IgE结合的表位。本研究根据表位疫苗方法学的原理，设计了BLG人IgE线性表位的串联序列(TBLG)，并在大肠杆菌中表达，制备了多克隆抗体(pAB-tBLG)。重组tBLG蛋白保留了表位的抗原性和潜在的致敏性。PAB-tBLG能识别tBLG的全部IgE表位，具有较高的特异性，但与酪蛋白的交叉反应性较弱。我们的结论是，Pab-tBLG可以检测出BLG，并可能用于乳制品中的过敏原残留的鉴定。
Bovine -lactoglobulin (BLG) is one of the major allergens in cow's milk. Epitopes play a key role in food allergy, while most of the antibodies developed do not specifically recognize the IgE-binding epitopes. Here, a tandem derived from human IgE linear epitopes of BLG (tBLG) was designed according to the principles of epitope-based vaccine methodology, expressed in Escherichia coli, and a polyclonal antibody (pAb-tBLG) was developed. The recombinant tBLG protein preserved the antigenicity and potential allergenicity of epitopes. The pAb-tBLG recognized all the IgE epitopes of tBLG, and showed a high specificity, but it had weak cross-reactivity with caseins. We concluded that pAb-tBLG can detect of BLG and may be useful for identifying allergenic residues in dairy products.