Evaluation of heat hyperalgesia and anxiety like-behaviors in a rat model of orofacial cancer
Evaluation of heat hyperalgesia and anxiety like-behaviors in a rat model of orofacial cancer
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DOI：
10.1016/j.neulet.2016.03.001
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发表时间：
2016-04-21
影响因子：
2.5
通讯作者：
Chichorro, Juliana Geremias
中科院分区：
文献类型：
--
作者：
Gambeta, Eder;Kopruszinski, Caroline Machado;Chichorro, Juliana Geremias
癌症患者通常会经历疼痛和焦虑，这两种情况都会严重影响他们的生活质量。一些作者声称疼痛和焦虑之间存在联系，而另一些人则认为没有直接联系。在任何情况下，确实有一个共识，即焦虑损害疼痛状况，超出了癌症疼痛患者的诊断和治疗不足。在此，我们研究了口面癌细胞接种诱导的大鼠热痛觉过敏是否会表现出焦虑样行为。此外，我们评估了疼痛阻断是否会导致焦虑行为的缓解，以及阻断焦虑是否会导致疼痛缓解。通过将步行者-256细胞接种到右侧触须垫中，在雄性Wistar大鼠中诱导口面癌。在接种后第6天评估热面部痛觉过敏，并在该时间点对大鼠进行高架十字迷宫和光暗转换测试。观察利多卡因和咪达唑仑对热痛敏和焦虑样行为的影响。癌细胞接种后6天检测到面部热痛敏高峰，此时大鼠表现出焦虑样行为增加。局部注射利多卡因（2%/50 μ L）可明显减轻热痛敏，但对焦虑样行为无影响，而咪唑安定（0.5mg/kg，i. p.）治疗未能改变热阈值，但诱导了抗焦虑样作用。总之，我们的数据表明，口面癌大鼠表现出疼痛和焦虑样行为，但短暂的热痛觉过敏缓解并不影响焦虑样行为，反之亦然，在我们的实验条件下。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Pain and anxiety are commonly experienced by cancer patients and both significantly impair their quality of life. Some authors claim that there is a relationship between pain and anxiety, while others suggest that there is not a direct association. In any case, there is indeed a consensus that anxiety impairs the pain condition beyond be under diagnosed and undertreated in cancer pain patients. Herein we investigated if rats presenting heat hyperalgesia induced by orofacial cancer cell inoculation would display anxiety-like behaviors. In addition, we evaluated if pain blockade would result in alleviation of anxiety behaviors, as well as, if blockade of anxiety would result in pain relief. Orofacial cancer was induced in male Wistar rats by inoculation of Walker-256 cells into the right vibrissal pad. Heat facial hyperalgesia was assessed on day 6 after the inoculation, and on this time point rats were submitted to the elevated plus maze and the light-dark transition tests. The influence of lidocaine and midazolam on heat hyperalgesia and anxiety like behaviors was assessed. The peak of facial heat hyperalgesia was detected 6 days after cancer cells inoculation, and at this time point, rats exhibited increased anxiety-like behaviors. Local treatment with lidocaine (2%/50 mu L) caused a marked reduction of heat hyperalgesia, but failed to affect the anxiety-like behaviors, while midazolam (0.5 mg/kg, i.p.) treatment failed to change the heat threshold, but induced an anxiolytic-like effect. Altogether, our data demonstrated that rats with orofacial cancer present pain and anxiety-like behaviors, but brief heat hyperalgesia relief does not affect the anxiety-like behaviors, and vice-versa, in our experimental conditions. (C) 2016 Elsevier Ireland Ltd. All rights reserved.