Expression, processing, and immunogenicity of the structural proteins of Venezuelan equine encephalitis virus from recombinant baculovirus vectors
Expression, processing, and immunogenicity of the structural proteins of Venezuelan equine encephalitis virus from recombinant baculovirus vectors
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DOI：
10.1016/s0264-410x(98)00335-1
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发表时间：
1999-03-05
期刊：
影响因子：
5.5
通讯作者：
Smith, JF
中科院分区：
文献类型：
--
作者：
Hodgson, LA;Ludwig, GV;Smith, JF
表达委内瑞拉马脑炎病毒（VEE）的结构蛋白的重组杆状病毒已被构建和表达产物的细胞内加工，抗原性和免疫原性进行了评估。表达完整结构蛋白区（C-E3-E2- 6 K-E1）或完整糖蛋白区（E3-E2- 6 K-E1）的杆状病毒在Sf 9细胞中产生的产物被准确和完全加工，并产生抗原性和免疫学上与真实病毒蛋白无法区分的成熟蛋白。这些产品在BALB/c小鼠中具有高度免疫原性，诱导有效的VEE中和反应，并保护这些动物免受强毒VEE的攻击。单个糖蛋白区域（E3-E2和6 K-E1）的表达产生了准确但不完全加工的产物，并诱导了与变性的VEE蛋白比天然的VEE蛋白更有效地反应的非中和抗体。尽管如此，用6 K-E1表达产物免疫提供了针对VEE攻击的完全保护。出版社：Elsevier Science Ltd
Recombinant baculoviruses expressing the structural proteins of Venezuelan equine encephalitis virus (VEE) have been constructed and the intracellular processing, antigenicity, and immunogenicity of the expression products have been assessed. Baculoviruses expressing the entire structural protein region (C-E3-E2-6K-E1), or the complete glycoprotein region (E3-E2-6K-E1), generated products in Sf9 cells that were accurately and completely processed, and resulted in mature proteins that were antigenically and electrophoretically indistinguishable from authentic viral proteins. These products were highly immunogenic in BALB/c mice, induced efficient VEE neutralizing responses, and protected these animals against challenge with virulent VEE. Expression of individual glycoprotein regions (E3-E2 and 6K-E1) generated products that were accurately but incompletely processed, and induced non-neutralizing antibodies that reacted more efficiently with denatured than native VEE proteins. Nonetheless, immunization with the 6K-E1 expression product provided complete protection against VEE challenge. Published by Elsevier Science Ltd.