Interstitial collagenase and the ED-B oncofetal domain of fibronectin are markers of angiogenesis in human skin tumors.
Interstitial collagenase and the ED-B oncofetal domain of fibronectin are markers of angiogenesis in human skin tumors.
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间质胶原酶和纤连蛋白的 ED-B 癌胎结构域是人类皮肤肿瘤中血管生成的标志物。
DOI：
10.1046/j.1525-1500.1998.00061.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Eisen,AZ
中科院分区：
文献类型：
--
作者：
Karelina,TV;Eisen,AZ
胶原酶-1（C1）是存在于新形成的微血管中的主要基质金属蛋白酶，并作为新生血管形成的标志物。纤维连接蛋白的癌胚片段（Fn-f）的表达被发现在血管生成过程中增加。在本研究中，我们研究了胶原酶-1和纤维连接蛋白的癌胚片段作为肿瘤血管生成的标志物在新形成的微血管中的表达之间的关系。在侵袭性皮肤肿瘤（即硬斑样和复发性基底细胞癌）和鳞状细胞癌中，新生血管与C1阳性和Fn-f阳性微血管数量的显著增加相关。在伸长开始时，微血管开始产生C1，但失去表达IV型胶原和FVIII相关抗原的能力。随后，内皮细胞产生Fn-f和C1。随着微血管的成熟，含C1的内皮细胞不能表达Fn-f，但开始产生含IV型胶原的基底膜和FVIII相关抗原。这些研究表明，未成熟内皮细胞选择性表达Fn-f和胶原酶。C1的产生开始于血管形成的早期阶段，并在整个血管生成过程中持续。相反，Fn-f表达仅限于血管发生的后期阶段，表明这些蛋白质是血管生成的可靠标志物。
Collagenase-1 (C1) is the predominant matrix metalloproteinase present in newly formed microvessels and serves as a marker of neovascularization. The expression of the oncofetal fragment of fibronectin (Fn-f) was found to be increased during angiogenesis. In the present study, we investigated the relationship between the expression of collagenase-1 and the oncofetal fragment of fibronectin in newly formed microvessels as markers of tumor angiogenesis. In aggressive skin tumors (ie, morpheaform and recurrent basal cell carcinomas) and squamous cell carcinomas, neovascularization was associated with a marked increase in the number of C1-positive and Fn-f-positive microvessels. At the beginning of elongation, microvessels begin to produce C1 but lose their ability to express type IV collagen and FVIII-related antigen. Later, this endothelium produces both Fn-f and C1. As maturation of microvessels occurs, C1-containing endothelium fails to express Fn-f but begins to produce a type IV collagen-containing basement membrane and FVIII-related antigen. These studies show that there is a selective expression of both Fn-f and collagenase by immature endothelial cells. C1 production begins at early stages of blood vessel formation and continues throughout angiogenesis. In contrast, Fn-f expression is limited to later stages of vasculogenesis, indicating that these proteins are reliable markers of angiogenesis.