Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize.
Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize.
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DOI：
10.1111/j.1574-6968.2006.00530.x
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发表时间：
2007
影响因子：
2.1
通讯作者：
Lixin Zhang;Guan-lin Xie
中科院分区：
文献类型：
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作者：
Lixin Zhang;Guan-lin Xie
对杭州市农田洋葱伯克霍尔德菌复合群（Bcc）进行了调查。在从水稻和玉米根际的选择性培养基上回收的251个细菌分离物中，通过PCR测定，其中112个被指定为Bcc。通过recA限制性片段长度多态性分析、种特异性PCR试验和recA基因测序的组合分析Bcc分离株的种组成。结果表明，大多数属于B。洋葱伯克霍尔德氏菌、新洋葱伯克霍尔德氏菌recA谱系IIIB、越南伯克霍尔德氏菌和吡咯伯克霍尔德氏菌。新洋葱伯克霍尔德氏菌和B.在玉米根际和水稻根际中，Bcc的种类组成和丰度差异较大，表明不同作物根际Bcc的种类组成和丰度差异较大。此外，一个分离物（R456）形成了一个单一的离散集群内的Bcc recA基因的系统发育分析，它可能属于一个新的基因型。
A survey of Burkholderia cepacia complex (Bcc) species was conducted in agricultural fields within Hangzhou, China. Out of the 251 bacterial isolates recovered on the selective media from the rhizosphere of rice and maize, 112 of them were assigned to Bcc by PCR assays. The species composition of the Bcc isolates was analyzed by a combination of recA-restriction fragment length polymorphism assays, species-specific PCR tests and recA gene sequencing. The results revealed that the majority belong to B. cepacia, Burkholderia cenocepacia recA lineage IIIB, Burkholderia vietnamiensis and Burkholderia pyrrocinia. Burkholderia cenocepacia and B. vietnamiensis dominated the rhizosphere of maize and rice, respectively, indicating that species composition and abundance of Bcc may vary dramatically in different crop rhizospheres. In addition, one isolate (R456) formed a single discrete cluster within the phylogenetic analysis of the Bcc recA gene, and it may belong to a new genomovar.