Two-photon polymerization nanofabrication of ultracompact light scattering spectroscopic probe for detection of pre-cancer in pancreatic cyst
Two-photon polymerization nanofabrication of ultracompact light scattering spectroscopic probe for detection of pre-cancer in pancreatic cyst
复制标题
双光子聚合纳米加工超小型光散射光谱探针检测胰腺囊肿癌前病变
DOI：
10.1016/j.optlaseng.2021.106616
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发表时间：
2021
影响因子：
4.6
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
--
作者：
Sheil, Conor J.;Khan, Umar;Zakharov, Yuri N.;Coughlan, Mark F.;Pleskow, Douglas K.;Sawhney, Mandeep S.;Berzin, Tyler M.;Cohen, Jonah M.;Glyavina, Maria;Zhang, Lei
胰腺癌是所有主要癌症中存活率最低的癌症之一，胰腺囊性病变占胰腺手术的三分之一。目前诊断胰腺囊肿恶性肿瘤的金标准是基于内镜超声引导下的细针穿刺（EUS-FNA）手术，其恶性肿瘤的检测准确率较低。在这里，我们展示了基于双光子聚合的折射和反射非接触式探针的设计和制造，能够快速监测整个内部囊肿表面，这是我们最近开发的接触式探针的一个进步，首次允许可靠地评估体内胰腺囊肿的恶性潜能。我们采用了一种新的双光子聚合技术，该技术允许直接激光写入精度达到数十纳米，以适应540微米内径的EUS-FNA针内的探针。这种新型探针能够很好地分离照明光束和收集光束，这对于空间门控方法的正确操作至关重要。这些探针在临床上可用于快速进行“光学活检”，最终消除良性囊肿不必要的胰腺手术和恶性囊肿手术切除的危险延误，改善患者预后和生活质量。
Pancreatic cancer has one of the worst survival rates of all major cancers, with pancreatic cystic lesions accounting for one in three pancreatic surgeries. The current gold-standard for diagnosis of pancreatic cyst malignancy is based on the endoscopic ultrasound guided fine-needle aspiration (EUS-FNA) procedure, which suffers from a low accuracy in detecting malignancy. Here we present the design and two-photon polymerization based fabrication of refractive and reflective non-contact probes, capable of rapid surveillance of the entire internal cyst surface-an advance over the contact probe we recently developed that allowed, for the first time, reliable evaluation of pancreatic cyst malignant potentialin vivo. We employed a novel two-photon polymerization technique, which allows direct laser-writing to an accuracy of tens of nanometers, to fit the probe within the 540 micrometer internal diameter EUS-FNA needle. The newly constructed probes show excellent separation of the illumination and collection beams, essential for proper operation of the spatial gating method. These probes can be used clinically to perform rapid “optical biopsy”, ultimately eliminating unnecessary pancreatic surgeries on benign cysts and dangerous delays in surgical removal of malignant cysts, improving patient prognosis and quality of life.