Further observations on the experimental production of scrapie in goats and sheep.
Further observations on the experimental production of scrapie in goats and sheep.
复制标题
山羊和绵羊痒病实验生产的进一步观察。
DOI：
10.1016/s0368-1742(60)80018-5
复制
发表时间：
1960
影响因子：
0.8
通讯作者：
G. C. Millson
中科院分区：
文献类型：
--
作者：
I. Pattison;G. C. Millson
56只山羊在脑悬液的5个脑道内均发生痒病。第五代潜伏期为8 ~ 11个月，第一代以羊脑为接种剂，潜伏期为15 ~ 22个月。在比较脑内（i/c）和皮下感染途径时，所有10只山羊在i/c感染后12-15个月出现瘙痒，10只山羊中有8只在s/c感染后21 -31个月出现瘙痒。但4只山羊给予非常大剂量的痒病脑悬液（10毫升，连续30或50天），仅在11-13个月内就出现了痒病。用核糖核酸酶或脱氧核糖核酸酶治疗，或通过普通邮件长途运输，都不影响痒病脑引起痒病的能力。2只山羊在瘙痒病潜伏期和临床阶段均未见直肠温度异常。5只患有天然痒病的斯瓦尔代母羊的脑悬液在接种i/c 14-29个月后，在25只切维奥特绵羊中有7只和10只山羊中有2只产生了该病。山羊瘙痒病的脑脊液和垂体、肾上腺、脾脏、胰腺和肝脏悬浮液在接种了i/c的山羊中引起病变，但甲状腺悬浮液没有引起病变。一只山羊在将健康山羊的大脑注射i/c后23个月患上了痒病。这只健康的山羊在7个月前用没有痒病病史的羔羊的血液进行了亚接种，这些羔羊之前接受了患有天然痒病的母羊所生羔羊的血液。与患有临床痒病的山羊保持接触24-34个月的28只山羊未发生痒病。
All of 56 goats developed scrapie in 5 intracerebral passages of brain suspension. The incubation period in the fifth passage was 8-11 months, compared with 15-22 months in the first passage when sheep brain was the inoculum. In a comparison of the intracerebral (i/c) and subcutaneous routes of infection, all of ten goats developed scrapie 12-15 months after i/c infection, and 8 of 10 in the 21st-31st months after s/c infection. But 4 goats given very large amounts of scrapie brain suspension s/c (10 ml. on 30 or 50 consecutive days) developed scrapie in only 11-13 months. The ability of scrapie brain to cause scrapie was not affected by treatment with ribonuclease or deoxyribonuclease, or by long distance transit by ordinary mail. No abnormal rectal temp, was recorded in 2 goats during the incubation and clinical stage of scrapie. Brain suspensions from 5 Swaledale ewes with natural scrapie produced the disease in 7 of 25 Cheviot sheep and 2 of 10 goats 14-29 months after i/c inoculation. Cerebro-spinal fluid and suspensions of pituitary gland, adrenal gland, spleen, pancreas and liver from goats with scrapie caused the disease in goats inoculated i/c, but thyroid suspension did not. A goat developed scrapie 23 months after i/c injection of brain from a healthy goat. This healthy goat had been sub-inoculated 7 months before with blood from lambs with no history of scrapie, which had previously received blood from lambs born to ewes with natural scrapie. Scrapie did not develop in 28 goats kept in contact for 24-34 months with goats having clinical scrapie.-M.G.G.