Registered report: Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs
Registered report: Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs
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DOI：
10.7554/elife.12470
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发表时间：
2016-03-01
期刊：
影响因子：
7.7
通讯作者：
Cook, Matthew
中科院分区：
文献类型：
--
作者：
Phelps, Mitch;Coss, Chris;Cook, Matthew
生殖项目：癌症生物学旨在通过对癌症生物学领域一些备受瞩目的论文中的选定实验进行复制，来解决人们对科学研究中可重复性日益增长的担忧。根据引文和Altmetric评分选择2010年至2012年期间发表的论文（Errington et at，2014）。该注册报告描述了Tay及其同事在2011年发表在Cell上的“Coding-Independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous 'mRNAs'”（Tay et al.，2011年）。待重复的实验是图3C、3D、3G、3 H、5A和5 B以及补充图3A和B中报道的那些。Tay及其同事提出了一种基于竞争性内源RNA（ceRNA）的新调控机制，该机制通过竞争性结合共享microRNA来调控靶基因。他们通过鉴定和确认靶向PTEN的ceRNA来测试他们的模型。在图3A和B中，他们报道了干扰推定的PTEN ceRNA的表达影响PTEN的表达。这种作用依赖于功能性微小RNA机制（图3G和H），并影响PTEN自身下游的途径（图5A和B）。生殖项目：癌症生物学是开放科学中心和科学交流中心之间的合作，复制结果将由eLife发布。
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high -profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et at, 2014). This Registered Report describes the proposed replication plan of key experiments from "Coding -Independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous 'mRNAs' by Tay and colleagues, published in Cell in 2011 (Tay et al., 2011). The experiments to be replicated are those reported in Figures 3C, 3D, 3G, 3H, 5A and 5B, and in Supplemental Figures 3A and B. Tay and colleagues proposed a new regulatory mechanism based on competing endogenous RNAs (ceRNAs), which regulate target genes by competitive binding of shared microRNAs. They test their model by identifying and confirming ceRNAs that target PTEN. In Figure 3A and B, they report that perturbing expression of putative PTEN ceRNAs affects expression of PTEN. This effect is dependent on functional microRNA machinery (Figure 3G and H), and affects the pathway downstream of PTEN itself (Figures 5A and B). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published by eLife.