Enhanced Shewanella biofilm promotes bioelectricity generation
Enhanced Shewanella biofilm promotes bioelectricity generation
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增强的希瓦氏菌生物膜促进生物发电
DOI：
10.1002/bit.25624
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发表时间：
2015-10-01
影响因子：
3.8
通讯作者：
Song, Hao
中科院分区：
文献类型：
--
作者：
Liu, Ting;Yu, Yang-Yang;Song, Hao
电活性生物膜在确定微生物燃料电池（MFC）的功率输出方面发挥着重要作用。为了工程化模型外电原Shewanella oneidensis MR-1的电活性生物膜形成，我们在S.oneidensis MR-1中异源过表达c-di-GMP生物合成基因ydeH，构建了其中ydeH的表达受IPTG诱导型启动子控制的突变菌株，以及其中ydeH受组成型启动子控制的菌株。这种工程化的希瓦氏菌菌株显着增强了生物膜的形成和生物电的产生。接种这些工程菌株的MFC实现了167.6+/-3.6mW/m(2)的最大功率密度，大约是野生型MR-1所达到的功率密度(61.0+/-1.9mW/m(2))的2.8倍。此外，生物电化学系统中的工程菌株在相对于饱和甘汞电极（SCE）的稳定电位为0.2V时产生了1100mA/m（2）的稳定电流密度，大约是野生型MR-1（320mA/m（2））的3.4倍。生物技术。生物工程。 2015；112：2051-2059。 (c) 2015 年 Wiley 期刊公司。
Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroacitve biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis MR-1, constructing a mutant strain in which the expression of ydeH is under the control of IPTG-inducible promoter, and a strain in which ydeH is under the control of a constitutive promoter. Such engineered Shewanella strains had significantly enhanced biofilm formation and bioelectricity generation. The MFCs inoculated with these engineered strains accomplished a maximum power density of 167.6 +/- 3.6mW/m(2), which was approximate to 2.8 times of that achieved by the wild-type MR-1 (61.0 +/- 1.9mW/m(2)). In addition, the engineered strains in the bioelectrochemical system at poised potential of 0.2V vs. saturated calomel electrode (SCE) generated a stable current density of 1100mA/m(2), approximate to 3.4 times of that by wild-type MR-1 (320mA/m(2)). Biotechnol. Bioeng. 2015;112: 2051-2059. (c) 2015 Wiley Periodicals, Inc.