Reactive astrocytes express bis, a Bcl-2-binding protein, after transient forebrain ischemia
Reactive astrocytes express bis, a Bcl-2-binding protein, after transient forebrain ischemia
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DOI：
10.1006/exnr.2002.7903
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发表时间：
2002-06-01
影响因子：
5.3
通讯作者：
Lee, JH
中科院分区：
文献类型：
--
作者：
Lee, MY;Kim, SY;Lee, JH
Bis（也称为Bag-3）是一种新的Bcl-2相互作用蛋白，已被证明可增强Bcl-2的抗细胞死亡活性。由于缺血/再灌注诱导Bcl-2的表达，我们研究了短暂前脑缺血后成年大鼠海马中Bis表达模式的变化。从海马提取的蛋白质的蛋白质印迹分析表明，与对照组相比，Bis的水平显着增加缺血后7天。免疫组化结果显示，Bis表达在CA 1区和齿状门区优先增加，并在再灌注后3-7天达到高峰。Bis和胶质细胞酸性蛋白（GFAP）表达的时间和空间模式非常相似，双免疫荧光组织化学显示，Bis在反应性星形胶质细胞中表达，表达GFAP。用抗Bcl-2和抗Hsp 70抗体对相邻切片进行免疫标记，结果显示，双免疫荧光组织化学证实了反应性星形胶质细胞中的Bis和Bcl-2模式。我们的研究结果表明，反应性星形胶质细胞短暂上调脑缺血/再灌注后，在成年大鼠海马。然而，Bis在星形胶质细胞反应中的确切作用有待确定。(C)2002 Elsevier Science（美国）。
Bis (also called Bag-3), identified as a novel Bcl-2-interacting protein, has been shown to enhance anti-cell death activity of Bcl-2. Because ischemia/reperfusion induces expression of Bcl-2, we examined the changes in the pattern of Bis expression in the adult rat hippocampus after transient forebrain ischemia. Western blot analysis with protein extracts from the hippocampus showed that, compared with controls, levels of Bis were markedly increased seven days after ischemia. An immunohistochemical study showed that the expression of Bis increased preferentially in the CA1 and the dentate hilar regions, and peaked at 3-7 days after reperfusion. The temporal and spatial patterns of expression for both Bis and glial fibrillary acidic protein (GFAP) were very similar, and double immunofluorescence histochemistry showed that Bis was expressed in reactive astrocytes, which express GFAP. Immunolabeling of adjacent sections with anti-Bcl-2 and anti-Hsp70 antibodies revealed that the pattern of Bis and Bcl-2 in reactive astrocytes was confirmed by double immunofluorescence histochemistry. Our results demonstrate that reactive astrocytes transiently up-regulate Bis after ischemia/reperfusion in the adult rat hippocampus. However, the precise role of Bis in the astrocytic response to be determined. (C) 2002 Elsevier Science (USA) .