Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.
Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.
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DOI：
10.2217/rme-2018-0063
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发表时间：
2019-02
影响因子：
2.7
通讯作者：
Sharada Paudel;Wen-han Lee;Moses Lee;T. Zahoor;R. Mitchell;Shang-You Yang;Haiqing Zhao;L. Schon-
中科院分区：
文献类型：
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作者：
Sharada Paudel;Wen-han Lee;Moses Lee;T. Zahoor;R. Mitchell;Shang-You Yang;Haiqing Zhao;L. Schon-
目的：本研究研究了利用循环多能基质细胞（MSC）振兴同种异体骨移植物的协调策略。材料和方法：经过趋化和释放评估后，将共聚物中的基质细胞衍生因子 1 和血小板衍生生长因子 BB 涂覆在同种异体骨移植物 (AlloS-P) 上。将单独涂有共聚物（Allo）的同种异体移植物作为对照，或将 AlloS-P 植入无胸腺小鼠的股骨中，在第 1、2 和 3 周接受人 MSC 或生理盐水静脉注射。结果：第 8 周时，同种异体移植物周围的总愈伤组织体积（软骨和骨愈伤组织）在 AlloS-P + MSC 组中最大（p < 0.05）。结论：用基质细胞衍生因子 1 和血小板衍生生长因子 BB 涂覆同种异体骨移植物并静脉注射 MSC，可改善同种异体移植物的融合。
Aim: This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs). Materials & methods: After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (AlloS-P). Allograft coated with copolymers alone (Allo), as controls, or AlloS-P was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3. Results: At week 8, the total callus volume (both cartilaginous and bony callus) around the allograft was the largest in the AlloS-P + MSC group (p < 0.05). Conclusion: Coating bone allograft with stromal cell-derived factor 1 and platelet-derived growth factor BB and intravenous injections of MSCs improved allograft incorporation.