Antitrypanosomal peptaibiotics, trichosporins B-VIIa and B-VIIb, produced by Trichoderma polysporum FKI-4452
Antitrypanosomal peptaibiotics, trichosporins B-VIIa and B-VIIb, produced by Trichoderma polysporum FKI-4452
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DOI：
10.1038/ja.2010.41
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Omura, Satoshi
中科院分区：
文献类型：
--
作者：
Iwatsuki, Masato;Kinoshita, Yuta;Omura, Satoshi
在筛选新的抗锥虫药物的过程中，本课题组发现并报道了几种新的生物活性天然化合物。1-3最近，我们从木霉的发酵液中分离到两个新的毛孢菌素类似物，分别命名为毛孢菌素B-VIIa(1)和B-VIIB(2)，以及5个已知的毛孢菌素(3-7)，4个。FKI-4452。这些化合物已被证明具有抗锥虫活性(图1)。本文介绍了这些新型毛孢菌素的发酵、分离、结构鉴定和生物活性。用稀释平板法从日本鹿儿岛八岛采集的土壤样品中分离到真菌菌株FKI-4452。利用ITSH(国际木霉和菌丝分类小组委员会)的TrichOKEY程序对菌株FKI-4452的ITS序列进行了测定并保存在日本DNA数据库中，登录号为AB517619，表明菌株FKI-4452属于多孢木霉。5.根据ITSHTrichoBLAST数据库，FKI-4452与多孢杆菌CBS 820.68的核苷酸序列有98%的同源性。5菌株FKI-4452经鉴定为多孢霉，定名为多孢木霉FKI-4452。目前尚无多孢霉菌CBS 820.68产毛孢菌素的报道。FKI-4452菌株在含有0.1%甘油、0.08%磷酸二氢钾、0.02%磷酸二氢钾、0.02%硫酸镁、0.02%氯化钾、0.2%硝酸钠、0.02%酵母膏和1.5%琼脂(灭菌前调节至pH 6.0)的LCA琼脂上生长和维持。木霉(Trichoderma sp.)将FKI-4452接种到100ml的种子培养基中，其中包括2.0%的葡萄糖，0.2%的酵母膏，0.5%的多肽(日本大阪Wako Pure Chemical Industries)，0.05%的硫酸镁，0.1%的磷酸二氢钾和0.1%的琼脂(调节pH
In the course of screening for new antitrypanosomal agents, several novel bioactive natural compounds have been discovered and reported by our group. 1–3 Recently, we have isolated two new trichosporin analogs, designated as trichosporins B-VIIa (1) and B-VIIb (2), together with five known trichosporins (3–7), 4 from the culture broth of Trichoderma sp. FKI-4452. These have proved to exhibit antitrypanosomal activity (Figure 1). In this paper, the fermentation, isolation, structure elucidation and biological activity of these novel trichosporins are described.Fungal strain FKI-4452 was isolated from a soil sample collected in Yakushima-Island, Kagoshima, Japan by the dilution plating method. The ITS sequence of strain FKI-4452 was determined and deposited at the DNA Data Bank of Japan, with the accession number AB517619, using The TrichOKEY program of ITSH (International Subcommission on Trichoderma and Hypocrea Taxonomy), which suggested that the strain FKI-4452 belongs to Trichoderma polysporum. 5 Moreover, FKI-4452 was a 98% match to the nucleotide sequences of T. polysporum CBS 820.68 according to the TrichoBLAST database of ITSH. 5 Thus, the strain FKI-4452 was identified as T. polysporum and designated Trichoderma polysporum FKI-4452. There was no report of trichosporin production by T. polysporum CBS 820.68. The strain FKI-4452 was grown and maintained on an LcA agar slant consisting of 0.1% glycerol, 0.08% KH2PO4, 0.02% K2HPO4, 0.02% MgSO4Á7H2O, 0.02% KCl, 0.2% NaNO3, 0.02% yeast extract and 1.5% agar (adjusted to pH 6.0 before sterilization). A loop of spores of Trichoderma sp. FKI-4452 was inoculated into 100 ml of the seed medium, which consisted of 2.0% glucose, 0.2% yeast extract, 0.5% Polypepton (Wako Pure Chemical Industries, Osaka, Japan), 0.05% MgSO4Á7H2O, 0.1% KH2PO4 and 0.1% agar (adjusted to pH